Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo
Bone marrow mesenchymal stem cells promote cell proliferation and neurotrophic function of Schwann cells in vitro and in vivo
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骨髓间充质干细胞在体外和体内促进雪旺细胞的细胞增殖和神经营养功能
DOI：
10.1016/j.brainres.2009.01.056
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发表时间：
2009-03-25
期刊：
影响因子：
2.9
通讯作者：
Gu, Xiaosong
中科院分区：
文献类型：
--
作者：
Wang, Jie;Ding, Fei;Gu, Xiaosong
骨髓间充质干细胞（MSCs）在神经组织工程中的应用可改善周围神经修复的功能结果。雪旺细胞（Schwann cells，SC）是周围神经系统的主要结构和功能细胞，在周围神经再生中起着重要作用。我们推测，MSC促进周围神经再生不仅通过其直接释放的神经营养因子，但通过间接调制的细胞行为的SC。为了验证我们的假设。采用体外共培养模型和大鼠坐骨神经再生的体内系统，观察MSCs对SCs增殖和神经营养因子表达的影响，通过细胞活力测定和流式细胞术、溴脱氧尿苷/Hoechst 33342双染色、免疫细胞/组织化学、RT-PCR和实时定量RT-PCR，以及Western blot分析共同证实了MSC对SC生物学特性的影响，特别是在周围神经再生期间。我们的研究结果有助于阐明骨髓间充质干细胞在周围神经修复中作为细胞治疗剂的作用机制。(C)2009 Elsevier B. V.保留所有权利。
The use of bone marrow-derived mesenchymal stem cells (MSCs) in nerve tissue engineering leads to an improved functional outcome of peripheral nerve repair. Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. We hypothesize that MSCs promote peripheral nerve regeneration not only via their direct release of neurotrophic factors, but through indirect modulation of cellular behaviors of SCs. To test this hypothesis we investigated. the influences of MSCs on proliferation of and neurotrophic factor expression by SCs using an in vitro co-culture model and an in vivo system of rat sciatic nerve regeneration, The data from cell viability assay and flow cytometry, bromodeoxyuridine/Hoechst 33342 double staining, immunocyto/histochemistry, RT-PCR and quantitative real-time RT-PCR, as well as Western blot analysis collectively confirmed the effects of MSCs on the biological characteristics of SCs, especially during the period of peripheral nerve regeneration. Our results help to elucidate the mechanisms by which MSCs function as a cell therapy agent in peripheral nerve repair. (C) 2009 Elsevier B.V. All rights reserved.