Spry is downregulated by multiple viruses to elevate ERK signaling and ensure viral reproduction in silkworm
Spry is downregulated by multiple viruses to elevate ERK signaling and ensure viral reproduction in silkworm
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Spry 被多种病毒下调，以增强 ERK 信号传导并确保病毒在家蚕中繁殖
DOI：
10.1016/j.dci.2019.04.001
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发表时间：
2019-09-01
影响因子：
2.9
通讯作者：
Jiang, Liang
中科院分区：
文献类型：
--
作者：
Guo, Huizhen;Sun, Qiang;Jiang, Liang
家蚕病毒性疾病主要由家蚕核型多角体病毒（BmNPV）、B.家蚕胞质型多角体病毒（BmCPV）和B.家蚕双腺病毒（BmBDV）。该病毒改变宿主细胞途径以促进其增殖。目前尚不清楚这三种家蚕病毒是否调节某种宿主途径。Spry是ERK上游的负调控因子。在本研究中，我们发现，在感染每种病毒后，家蚕中BmSpry降低，p-ERK升高。构建了BmSpry转基因RNAi载体，并将其用于胚胎显微注射，获得了转基因株系Spry-1。与未转基因家蚕相比，BmSpry在Spry-I中的表达显著降低。三种病毒感染后，Spry-I的病毒含量和死亡率均显著高于非转基因幼虫。与非转基因对照相比，病毒感染后Spry-I中的p-ERK增加。这些结果表明，BmSpry被多种不同类型的病毒下调，以提高p-ERK并确保家蚕中的病毒繁殖。
Viral diseases of silkworm are mainly caused by Bombyx mori nucleopolyhedrovirus (BmNPV), B. mori cytoplasmic polyhedrosis virus (BmCPV) and B. mori bidensovirus (BmBDV). The virus alters host cellular pathways to facilitate its proliferation. It is still unclear whether the three silkworm viruses regulate a certain host pathway. Spry is a negative regulator upstream of ERK In this study, we found that BmSpry was decreased and p-ERK was increased in silkworm after infection with each virus. A transgenic RNAi vector of BmSpry was constructed and used for embryo microinjection to generate the transgenic line Spry-I. The expression of BmSpry was significantly reduced in Spry-I compared to that in non-transgenic silkworm. The viral content and mortality in Spry-I were significantly higher than those in non-transgenic larvae after infection with the three viruses. p-ERK was increased in Spry-I compared to that in non-transgenic control after virus infection. These results suggest that BmSpry is downregulated by multiple different classes of viruses to elevate p-ERK and ensure viral reproduction in the silkworm.