A new perfusion chamber to detect platelet adhesion using a small volume of blood
A new perfusion chamber to detect platelet adhesion using a small volume of blood
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DOI：
10.1016/s0049-3848(98)00159-5
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发表时间：
1998-12-15
影响因子：
7.5
通讯作者：
IJsseldijk, M
中科院分区：
文献类型：
--
作者：
Sixma, JJ;de Groot, PG;IJsseldijk, M
人血灌注研究传统上在相对较大的腔室（10 × 0.6 mm）中进行，血液以55 mL/min的速度循环通过该腔室，以实现剪切速率（s.r.）1600/sec。有时使用15 ml的体积，这意味着在5分钟的标准灌注期间，血液通过腔室再循环多达20次。我们开发了一种尺寸小得多的新腔室（5x0.1 mm），通过该腔室，使用注射泵以0.3 mL/min的速度抽取血液进行s.r. 1600/sec。该腔室具有在灌注期间很少发生血小板活化的优点。需要较少量的抑制剂或抗体，并且可以使用来自单个患者的天然血液进行多次灌注。介绍了该系统的组成及优缺点。对同一腔室进行了修改，用于直接观察和定量流动下中性粒细胞和单核细胞与血小板和/或纤维蛋白的粘附。流动下的血小板粘附也已经使用该相同的腔室通过直接观察进行了研究。(C)1998 Elsevier Science Ltd.
Perfusion studies with human blood are traditionally performed in relatively large chambers (10x0.6 mm) through which blood is circulated at 55 mL/min to achieve a shear rate (s.r.) of 1600/sec. A volume of 15 mi is sometimes used which means the blood is recirculated up to 20 times through the chamber during a standard perfusion of five minutes. We have developed a new chamber of much smaller dimensions (5x0.1 mm) through which blood is drawn with a syringe pump at a velocity of 0.3 mL/min for a s.r. of 1600/sec. This chamber has the advantage that little platelet activation occurs during perfusion. Smaller quantities of inhibitors or antibodies are required and it is possible to perform several perfusions with native blood from a single patient. The setup, advantages and disadvantages of the system are described. A modification of the same chamber has been used for the direct observation and quantitation of the adhesion of neutrophils and monocytes to platelets and/or fibrin under flow. Platelet adhesion under flow has also been studied with direct observation using this same chamber. (C) 1998 Elsevier Science Ltd.