Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model
Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model
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DOI：
10.1002/jbm.a.30579
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发表时间：
2006-06-15
影响因子：
4.9
通讯作者：
Kim, Byung-Soo
中科院分区：
文献类型：
--
作者：
Kang, Sun-Woong;Son, Sun-Mi;Kim, Byung-Soo
目前膝关节半月板损伤的治疗方法尚不能完全预防半月板损伤的副作用。半月板组织工程是治疗半月板损伤的一种新方法。本研究应用组织工程技术在兔半月板全切除模型上再生整个内侧半月板。生物可降解支架的形状是由聚乙醇酸（PGA）纤维网制成的，该纤维网通过在交叉点用75：25聚（乳酸-共-乙醇酸）粘合PGA纤维而机械增强。结合的PGA支架的压缩模量比未结合的支架高28倍。从兔半月板活检组织中分离同种异体半月板细胞并进行体外培养。将扩增后的兔膝关节软骨细胞接种于聚合物支架上，体外培养1周，移植于去除内侧滑膜的兔膝关节。移植后10周或36周，植入物形成新生半月板，基本保持原支架形状。6周和10周新生半月板切片苏木精-伊红染色显示纤维软骨再生。番红-O染色显示10周时新生半月板中有大量蛋白多糖。Masson三色染色表明胶原蛋白的存在。免疫组织化学分析显示，10周时新生半月板中I型和11型胶原的存在与正常组织相似。在36周时对组织工程化骨进行生物化学和生物力学分析，以确定组织工程化骨的质量。与自体半月板相比，组织工程半月板的胶原蛋白含量和聚集体模量存在差异。本研究首次证明了利用组织工程方法在兔全髋关节切除模型中再生整个关节软骨的可行性。(c)2006 Wiley Periodicals，Inc.
The current treatments of meniscal lesion in knee joint are not perfect to prevent adverse effects of meniscus injury. Tissue engineering of meniscus using meniscal cells and polymer scaffolds could be an alternative option to treat meniscus injury. This Study reports on the regeneration of whole medial meniscus in a rabbit total meniscectomy model using the tissue engineering technique. Biodegradable scaffolds in a meniscal shape were fabricated from poly,glycolic acid (PGA) fiber meshes that were mechanically reinforced by bonding PGA fibers at cross points with 75:25 poly(lactic-co-glycolic acid). The compressive modulus Of the bonded PGA scaffold was 28-fold higher than that of nonbonded scaffold. Allogeneic meniscal cells were isolated from rabbit meniscus biopsy and Cultured in vitro. The expanded meniscal cells were seeded onto the polymer scaffolds, cultured in vitro for 1 week, and transplanted to rabbit knee joints from which medial menisci were removed. Ten or 36 weeks after transplantation, the implants formed neomenisci with the original scaffold shape maintained approximately. Hematoxylin and eosin staining of the sections of the neomenisci at 6 and 10 weeks revealed the regeneration of fibrocartilage. Safranin-O staining showed that abundant proteoglycan was present in the neomenisci at 10 weeks. Masson's trichrome staining indicated the presence of collagen. Immunohistochemical analysis showed that the presence of type I and 11 collagen in neomenisci at 10 weeks was similar to that of normal meniscal tissue. Biochemical and biomechanical analyses of the tissue-engineered menisci at 36 weeks were performed to determine the quality of the tissue-engineered menisci. Tissue-engineered meniscus showed differences in collagen content and aggregate modulus in comparison with native meniscus. This study demonstrates, for the first time, the feasibility of regenerating whole meniscal cartilage in a rabbit total meniscectomy model using the tissue engineering method. (c) 2006 Wiley Periodicals, Inc.