Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
复制标题
使用高通量 RNA 测序分析 EBV 转录。
DOI:
10.1007/978-1-4939-6655-4_7
复制
发表时间:
2017
期刊:
影响因子:
--
通讯作者:
Flemington,ErikK
中科院分区:
文献类型:
--
作者:
O'Grady,Tina;Baddoo,Melody;Flemington,ErikK
High-throughput sequencing of RNA is used to analyze the transcriptomes of viruses and cells, providing information about transcript structure and abundance. A wide array of programs and pipelines has been created to manage and interpret the abundance of data generated from high-throughput RNA sequencing experiments. This protocol details the use of free and open-source programs to align RNA-Seq reads to a reference genome, visualize read coverage and splice junctions, estimate transcript abundance, and evaluate differential expression of transcripts in different conditions. Particular concerns related to EBV and viral transcriptomics are addressed and access to EBV reference files is provided.
DOI:
10.1007/978-1-60761-944-4_10
发表时间:
2011-01-01
期刊:
PCR PROTOCOLS, THIRD EDITION
影响因子:
--
作者:
Kurosawa, Junpei;Nishiyori, Hiromi;Hayashizaki, Yoshihide
通讯作者:
Hayashizaki, Yoshihide
影响因子:
6.7
作者:
Arias C;Weisburd B;Stern-Ginossar N;Mercier A;Madrid AS;Bellare P;Holdorf M;Weissman JS;Ganem D
通讯作者:
Ganem D