Flexible, Graphene-Coated Biocomposite for Highly Sensitive, Real-Time Molecular Detection
Flexible, Graphene-Coated Biocomposite for Highly Sensitive, Real-Time Molecular Detection
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用于高灵敏度、实时分子检测的柔性石墨烯涂层生物复合材料
DOI：
10.1002/adfm.201603550
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发表时间：
2016-12-20
影响因子：
19
通讯作者：
Cho, Nam-Joon
中科院分区：
文献类型：
--
作者：
Wang, Lili;Jackman, Joshua A.;Cho, Nam-Joon
可穿戴生物传感器在医疗保健和环境应用方面具有巨大的潜力，而开发灵活和生物兼容的传感平台来高精度检测生理生物标志物仍然是一个难以实现的目标。本文描述了一种超灵敏的柔性传感器，该传感器基于三维分层生物复合材料，该复合材料由中空的天然花粉微胶囊组成，并涂有导电石墨烯层。石墨烯涂层微胶囊的模块化组装到超薄聚对苯二甲酸乙二醇酯层上，使得高度灵活的传感器配置具有可调的选择性，随后对目标抗原的抗体进行共价固定。在概念验证示例中，该生物传感器展示了对前列腺特异性抗原（PSA）的超高灵敏度检测，低至1.7 x 10(-15) M，具有实时反馈和优于传统二维石墨烯涂层传感器的性能。重要的是，该器件在各种弯曲条件下的性能始终很高。综上所述，这项工作的结果突出了采用轻质生物复合材料作为模块化构建模块的优点，用于设计具有高响应和敏感分子检测能力的柔性生物传感器。
Wearable biosensors hold significant potential for healthcare and environmental applications, and the development of flexible and biocompatible sensing platforms for high accuracy detection of physiological biomarkers remains an elusive goal. Herein, an ultrasensitive, flexible sensor is described that is based on a 3D hierarchical biocomposite comprised of hollow, natural pollen microcapsules that are coated with a conductive graphene layer. Modular assembly of the graphene-coated microcapsules onto an ultrathin polyethylene terephthalate layer enables a highly flexible sensor configuration with tunable selectivity afforded by subsequent covalent immobilization of antibodies against target antigens. In a proof-of-concept example, the biosensor demonstrates ultrahigh sensitivity detection of prostate specific antigen (PSA) down to 1.7 x 10(-15) M with real-time feedback and superior performance over conventional 2D graphene-coated sensors. Importantly, the device performance is consistently high across various bending conditions. Taken together, the results demonstrated in this work highlight the merits of employing lightweight biocomposites as modular building blocks for the design of flexible biosensors with highly responsive and sensitive molecular detection capabilities.