Transcription pattern of UL131A-128 mRNA in clinical strains of human cytomegalovirus
Transcription pattern of UL131A-128 mRNA in clinical strains of human cytomegalovirus
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DOI：
10.1007/s12038-010-0041-3
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发表时间：
2010-07
影响因子：
2.9
通讯作者：
Zhengrong Sun;G. Ren;Yan-Pin Ma;Ning Wang;Yao-hua Ji;Y. Qi;Mali Li;R. He;Q. Ruan
中科院分区：
文献类型：
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作者：
Zhengrong Sun;G. Ren;Yan-Pin Ma;Ning Wang;Yao-hua Ji;Y. Qi;Mali Li;R. He;Q. Ruan
从人巨细胞病毒（HCMV）临床分离株感染的人胚肺成纤维细胞中获得HCMV mRNA。采用逆转录-聚合酶链反应（RT-PCR）扩增UL 131 A-128 mRNA的cDNA，并通过测序分析。同时，在临床株的HCMV cDNA文库中选择含有UL 131 A-128转录本的克隆并测序。结果表明，UL 131 A-128 mRNA的表达具有立即早期、早期和晚期动力学。RT-PCR结果表明，UL 131 A基因由两个外显子组成，编码区不存在内含子。然而，UL 128基因的转录本显示出两种模式：一种模式由三个外显子组成，如前所述;另一种模式包含三个外显子和第一个内含子。此外，从HCMV cDNA文库中选择的克隆的序列证实了UL 131 A-128剪接转录本的上述特征。我们的结果表明，UL 131 A，UL 130和UL 128基因的转录与3 '-共末端，虽然它们的mRNA的起始点可能是不同的。我们的研究中发现的转录本的变异表明了HCMV临床株中UL 131 A-128基因转录的复杂性。
Human cytomegalovirus (HCMV) mRNA was obtained from human embryonic lung fibroblast cells infected by HCMV clinical strains from urine samples of infants at different kinetic periods. The cDNA of UL131A-128 mRNAs was amplified using reverse transcription-polymerase chain reaction (RT-PCR) and analysed by sequencing. Meanwhile, clones containing UL131A-128 transcripts in an HCMV cDNA library of a clinical strain were selected and sequenced. It was demonstrated that UL131A-128 mRNA was expressed with immediately early, early and late kinetics. Sequences obtained by RT-PCR showed that the UL131A gene consisted of two exons and the coding region of the UL130 gene was not interrupted by any intron in the region as reported earlier. However, the transcript of the UL128 gene showed two patterns: one pattern consisted of three exons as reported earlier; the other contained the three exons and also the first intron. Moreover, the above characteristics of UL131A-128 spliced transcripts were confirmed by the sequences of clones selected from the HCMV cDNA library. Our results demonstrated that the UL131A, UL130 and UL128 genes were transcribed with the 3'-coterminal, although the initiation points of their mRNA may be different. The variation in the transcripts found in our study indicated the complex nature of transcription of UL131A-128 genes in clinical strains of HCMV.