Wakame and Nori in Restructured Meats Included in Cholesterol-enriched Diets Affect the Antioxidant Enzyme Gene Expressions and Activities in Wistar Rats
Wakame and Nori in Restructured Meats Included in Cholesterol-enriched Diets Affect the Antioxidant Enzyme Gene Expressions and Activities in Wistar Rats
复制标题
富含胆固醇的饮食中包含的重组肉中的裙带菜和紫菜影响 Wistar 大鼠的抗氧化酶基因表达和活性
DOI：
10.1007/s11130-010-0179-z
复制
发表时间：
2010
影响因子：
4
通讯作者：
F. Sánchez
中科院分区：
文献类型：
--
作者：
Adriana R. Schultz Moreira;L. González;Raúl Olivero;S. Bastida;J. Benedí;F. Sánchez
研究了含裙带菜或紫菜的重组肉（RM）对肝脏总谷胱甘肽状态和几种抗氧化酶基因表达和活性的影响。6组雄性Wistar大鼠，每组10只，喂食85% AIN-93 M饲料和15%冻干RM的混合物35天。对照组（C）食用对照RM、Wakame（W）和Nori（N）组，RM分别含有5% Wakame和5% Nori。添加胆固醇饲料（CC、CW和CN）的动物食用添加胆固醇（2%）和胆酸（0.4%）的相应基础饲料。藻类和膳食胆固醇显著相互作用（P< 0.002），影响所有酶的表达，但不影响活性。胆固醇补充剂降低了大多数酶的表达和活性。W-RM组与C-RM组相比，GPx、GR、Mn-SOD、Cu，Zn-SOD的表达均增加（P< 0.05），过氧化氢酶的表达降低（P< 0.05）。N-RM组与C-RM组相比，过氧化氢酶和Mn-SOD的表达增加（P< 0.05）。W-RM组GR活性升高，N组SOD活性升高，Se-GPx活性降低。W-RM增加总谷胱甘肽和还原型谷胱甘肽，降低氧化还原指数。CN饮食诱导的血浆胆固醇水平显著低于CW饮食（P< 0.001）。总之，Nori-RM是一种低胆固醇食物，而Wakame-RM是一种抗氧化食物。在将这类RM作为潜在的人类功能性食品时，应考虑到这一点。
The effects of diets including restructured meats (RM) containing Wakame or Nori on total liver glutathione status, and several antioxidant enzyme gene expressions and activities were tested. Six groups of ten male growing Wistar rats each were fed a mix of 85% AIN-93 M diet and 15% freeze-dried RM for 35 days. The control group (C) consumed control RM, the Wakame (W) and the Nori (N) groups, RM with 5% Wakame and 5% Nori, respectively. Animals on added cholesterol diets (CC, CW, and CN) consumed their corresponding basal diets added with cholesterol (2%) and cholic acid (0.4%). Alga and dietary cholesterol significantly interact (P< 0.002) influencing all enzyme expressions but not activities. The cholesterol supplement decreased most enzyme expression and activity. W-RMvs.C-RM increased (P< 0.05) expression of GPx, GR, Mn-SOD, and Cu,Zn-SOD and decreased that of catalase. N-RMvs.C-RM increased (P< 0.05) expression of catalase and Mn-SOD. GR activity increased in W-RM rats while SOD activity increased, but that of Se-GPx decreased in N animals. W-RM increased total and reduced glutathione and decreased the redox index. CN diet induced significantly lower plasma cholesterol levels (P< 0.001) than the CW diet. In conclusion, Nori-RM is a hypocholesterolemic food while Wakame-RM is an antioxidant food. This should be taken into account when including this kind of RM as potential functional foods in human.
长期接触无机砷酸盐会降低兔子肝脏硫氧还蛋白还原酶和谷胱甘肽还原酶的酶活性。
DOI：
--
发表时间：
2003
期刊：
Environmental Toxicology 18
影响因子：
--
作者：
Nikaido M;Pi JB;Kumagai Y;Yamauchi H;Taguchi K;Horiguchi S;Sun Y;Sun GF;Shimojo N
通讯作者：
Shimojo N