TOXICOKINETICS OF 2,4-DIHYDROXY-7-METHOXY-1,4-BENZOXAZIN-3-ONE (DIMBOA) IN THE EUROPEAN CORN-BORER, OSTRINIA-NUBILALIS (HUBNER)
TOXICOKINETICS OF 2,4-DIHYDROXY-7-METHOXY-1,4-BENZOXAZIN-3-ONE (DIMBOA) IN THE EUROPEAN CORN-BORER, OSTRINIA-NUBILALIS (HUBNER)
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DOI：
10.1007/bf01207432
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发表时间：
1989-07-01
影响因子：
2.3
通讯作者：
TIMMINS, G
中科院分区：
文献类型：
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作者：
CAMPOS, F;ATKINSON, J;TIMMINS, G
合成了玉米中主要的羟肟酸- 2,4-二羟基-7-甲氧基-1,4-苯并恶嗪-3-one （DIMBOA）及其氚化衍生物，用于测定该杀虫剂对欧洲玉米螟（ECB）的毒代动力学。在添加DIMBOA（0、0.05、0.2和0.5 mg/g饲粮）的生长研究中，随着浓度的增加，平均化蛹时间和成虫羽化时间显著延长。雌雄蛹和成虫体重均随浓度的增加而降低。浓度高时，幼虫和蛹的死亡率增高。在0.2和0.5 mg/g饲粮中，DIMBOA导致每只雌鱼的产蛋量减少，而在0.5 mg/g饲粮中，每颗卵的产蛋量减少。从初生期开始，添加0.2 mg [3H]- + [1H]DIMBOA/g的日粮，4 ~ 5龄时，标签含量呈上升趋势，但在化蛹和羽化时，标签含量下降。大量标记的化合物在蛹壳中被昆虫排出。在剂量相关研究中，摄取和排泄都随着DIMBOA浓度的增加而增加（0.05、0.2、0.4 mg/g日粮），而在所有浓度下，身体负荷（组织中浓度/草中浓度）保持在0.25左右。在DIMBOA的最高剂量（0.4 mg/g）下，欧洲央行增加了消耗量，可能是为了补偿该化合物的毒性作用。在局部应用研究中，标记化合物在草中的消除速度很快，4小时达到65%，48小时达到88%。除血淋巴外，组织中标签的积累较少。结果表明，ECB确实具有适应机制来处理这种宿主衍生化合物的影响。
2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), the major hydroxamic acid present in corn, and its tritiated derivative, were prepared synthetically for use in the determination of the toxicokinetics of this insect deterrent in the European corn borer (ECB), Ostrinia nubilalis. In growth studies with DIMBOA (0, 0.05, 0.2, and 0.5 mg/g diet), the mean time to pupation and adult emergence were significantly lengthened by an increase in concentration. Pupal and adult weights, for both female and male, decreased with an increase in concentration. Increased larval and pupal mortality occurred at the highest concentration of DIMBOA. DIMBOA, at concentrations of 0.2 and 0.5 mg/g diet, resulted in a decrease in the number of egg masses produced per female, and at 0.5 mg/g diet, in a decrease in the number of eggs per egg mass. Larvae fed from the neonate stage on a diet containing 0.2 mg [3H]- + [1H]DIMBOA/g diet showed an increase in the content of label from fourth to fifth instar, but levels declined at pupation and emergence. A large amount of the labeled compounds was excreted by the insect in the pupal case. In dose-related studies, both uptake and excretion incresed with an increase in concentration of DIMBOA (0.05, 0.2, 0.4 mg/g diet), while a body burden (concentration in the tissues/concentration in the frass) of approximately 0.25 was maintained for all concentrations. At the highest dose of DIMBOA (0.4 mg/g), the ECB increased consumption, possibly to compensate for the toxic effects of the compound. In topical application studies, elimination of the labeled compound in the frass was rapid, reaching 65% by 4 hr and 88% for 48 hr. Accumulation of label in tissues other than hemolymph was low. The results show that the ECB does possess adaptive mechanisms to deal with the effects of this host-derived compound.