Production of inbred offspring by intracytoplasmic sperm injection of oocytes from juvenile female mice
Production of inbred offspring by intracytoplasmic sperm injection of oocytes from juvenile female mice
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幼年雌性小鼠卵母细胞胞浆内单精子注射产生近交后代
DOI：
10.1071/rd16399
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发表时间：
2018-02-01
影响因子：
1.9
通讯作者：
Dai, Yan-Feng
中科院分区：
文献类型：
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作者：
Zhu, Jie;Cui, Wei;Dai, Yan-Feng
本研究的目的是确定使用幼年雌性小鼠的卵母细胞是否会提高卵胞浆内单精子注射（ICSI）的效率。本研究对15只成年和14只幼年C57 BL 6/J雌性小鼠进行超数排卵，成年小鼠每只获得17.8个卵母细胞，显著低于幼年小鼠的40.2个卵母细胞（P0.05）。注射人绒毛膜促性腺激素后12 h，取C57 BL/6 J小鼠卵母细胞，置于KSOM中，随机分组，分别于0、2、4、6 h用上述方法激活，然后在KSOM中培养3.5d。各组间桑椹胚率和囊胚率差异不显著（P>0.05）。用10 mM SrCl 2激活2 h的小鼠卵母细胞进行ICSI，原核形成率和第1天卵裂率均显著高于对照组（P
The aim of the present study was to determine whether the use of oocytes from juvenile female mice would improve the efficiency of intracytoplasmic sperm injection (ICSI). In the present study, 15 adult and 14 juvenile C57BL6/J female mice were superovulated, with 17.8 oocytes per mouse harvested from adults, significantly lower than the 40.2 harvested from juveniles (P0.05). Twelve hours after injection of human chorionic gonadotrophin, oocytes were harvested from C57BL/6J juvenile mice into KSOM, randomly divided into groups and activated with the same method mentioned above at 0, 2, 4 or 6 h and then cultured in KSOM for 3.5 days. There was no significant difference in morula and blastocyst rates among the different groups (P>0.05). Oocytes from juvenile mice activated in 10 mM SrCl2 for 2 h were subjected to ICSI and the rates of pronuclear formation and Day 1 cleavage were significantly improved compared with the control group (P