Effects of repeated oral postnatal exposure to chlorpyrifos on open-field behavior in juvenile rats
Effects of repeated oral postnatal exposure to chlorpyrifos on open-field behavior in juvenile rats
复制标题
DOI：
10.1093/toxsci/59.2.260
复制
发表时间：
2001-02-01
影响因子：
3.8
通讯作者：
Chambers, JE
中科院分区：
文献类型：
--
作者：
Carr, RL;Chambers, HW;Chambers, JE
有机磷（OP）杀虫剂有可能对儿童造成行为影响。本研究旨在确定是否重复口服暴露于毒死蜱的断奶前大鼠会产生行为的变化，在断奶前和断奶后的年龄。从出生后第1天（PND）开始，每隔一天给药一次，并持续至PND 21。大鼠接受以下方案之一：从PND 1-21开始低剂量（3 mg/kg）;从PND 1-5开始中等剂量（mg/kg），然后从PND 7-21开始6 mg/kg;或从PND 1-5开始3 mg/kg，然后从PND 7-13开始6 mg/kg，从PND 1 - 5 -21开始12 mg/kg的高剂量方案。对照组、低剂量组和中剂量组之间的体重无差异，但高剂量组在PND 13-21时体重显著降低。在PND 10、12、14、16、18、20、25和30时，使用开放视野测量自发活动。雄性和雌性之间的自发活动水平或给药效应无差异。在PND 10、12、14、16、18和20，任何剂量均对自发活动无影响。在第25天和第30天，中剂量组和高剂量组的自发活动显著减少。脑胆碱酯酶（ChE）抑制在PND 25时约为25-38%，在PND 30时约为14-34%。在PND 25而非30，肺和膈肌ChE和血清丁酰胆碱酯酶（BChE），与高剂量组的动物，和心脏ChE与中，高剂量组显着抑制。在PND 25和30时，骨骼肌ChE和血清乙酰胆碱酯酶（AChE）无明显抑制。这些数据表明，出生后早期毒死蜱暴露会抑制幼年大鼠的运动活动，脑胆碱酯酶活性基本恢复后的影响最为明显。
Organophosphorus (OP) insecticides have the potential to cause behavioral effects in children. This study was designed to determine if repeated oral exposure of preweanling rats to chlorpyrifos would produce behavioral changes at both pre- and postweanling ages. Treatment occurred every second day beginning on postnatal day (PND) 1, and continued through PND 21. The rats received one of the following regimens: a low-dosage (3 mg/kg) from PND 1-21; a medium dosage (mg/kg from PND 1-5, and then 6 mg/kg from PND 7-21; or a high-dosage schedule of 3 mg/kg on PND 1-5, then 6 mg/kg from PND 7-13, and 12 mg/kg from PND 15-21. There were no differences in body weights among the control-, low-, and medium-dosage groups but the high-dosage group had significantly lower body weights on PND 13-21. An open field was used to measure locomotor activity on PND 10, 12, 14, 16, 18, 20, 25, and 30. There were no differences in locomotor activity levels or treatment effects between males and females. On PND 10, 12, 14, 16, 18, and 20 there was no effect on locomotor activity with any dosage. On days 25 and 30, locomotor activity was significantly decreased with the medium- and high-dosage groups. Brain cholinesterase (ChE) inhibition mas about 25-38% on PND 25 and 14-34% on PND 30. On PND 25 but not 30, lung and diaphragm ChE and serum butyrylcholinesterase (BChE), with the high-dosage animals, and heart ChE with the medium- and high-dosage groups were significantly inhibited. There was no significant inhibition of skeletal muscle ChE or serum acetylcholinesterase (AChE) on PND 25 and 30. These data suggest that early postnatal chlorpyrifos exposures will depress locomotor activity in juvenile rats, with the effects most pronounced after brain ChE activity has substantially recovered.