Zinc protoporphyrin level in blood determined by a portable hematofluorometer: a screening device for lead poisoning.
Zinc protoporphyrin level in blood determined by a portable hematofluorometer: a screening device for lead poisoning.
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通过便携式血液荧光计测定血液中锌原卟啉水平：一种铅中毒筛查装置。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
David M. Zuckerman
中科院分区：
文献类型：
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作者：
W. Blumberg;J. Eisinger;Lamola Aa;David M. Zuckerman
设计了一种廉价的便携式荧光计，可用于测定沉积在一次性载玻片上的一滴未处理的血液中锌原卟啉（ZPP）的浓度。无需测量血样的体积。该仪器准确、可重复地显示血液中的 ZPP 水平（以微克/分升为单位），通过将血液荧光与稳定染料的荧光进行比较来自动校准每次测定。该仪器被我们称为血液荧光计，非常适合用于儿童和职业铅中毒的初级筛查。公共卫生人员经过几分钟的指导后即可使用。比较了使用血液荧光计获得的 ZPP 水平与使用公认的提取方法获得的红细胞原卟啉水平。
An inexpensive portable fluorometer has been designed which can be used to determine the concentration of zinc protoporphyrin (ZPP) in a drop of unprocessed blood deposited on a disposable glass slide. No volume measurement of the blood sample need be made. The instrument displays the ZPP level in micrograms per deciliter of blood accurately and reproducibly, automatically calibrating each determination by comparing the blood fluorescence with that of a stable dye. The instrument, which we call a hematofluorometer, is ideally suited for use in primary screening for childhood and occupational lead intoxication. It can be used by public health personnel after a few minutes of instruction. Comparisons of ZPP levels obtained with the use of the hematofluorometer with erythrocyte protoporphyrin levels obtained using accepted extraction methods are presented.