Caprin controls follicle stem cell fate in the Drosophila ovary.
Caprin controls follicle stem cell fate in the Drosophila ovary.
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DOI：
10.1371/journal.pone.0035365
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Papoulas O
中科院分区：
文献类型：
--
作者：
Reich J;Papoulas O
成体干细胞必须平衡自我更新和分化以实现组织稳态。果蝇卵巢提供了有关确保适当的生殖干细胞更新和分化所需的外在生态位信号和内在分子过程的丰富信息。最近发现的控制卵巢卵泡干细胞行为的因素尚不清楚，但对生育能力同样重要。在这里，我们报告翻译调节因子在控制这些细胞中发挥着关键作用。具体来说，卵泡干细胞谱系中需要翻译调节因子 Caprin (Capr)，以确保该干细胞群的维持以及卵泡干细胞后代对发育中的生殖细胞的正确封装。此外，编码翻译调节因子脆性X智力迟钝蛋白的基因fmr1的一个拷贝的减少加剧了Capr封装表型，表明Capr和fmr1正在调节一个共同的过程。山羊蛋白之前在脊椎动物中被描述为细胞质激活/增殖相关蛋白。值得注意的是，我们发现 Caprin 的缺失会改变细胞周期的动态，并且我们提供的证据表明 CycB 的失调会导致卵泡干细胞后代行为的破坏。我们的研究结果支持这样的观点，即翻译调节因子可能提供一种保守的机制来监督发育关键的细胞周期，例如干细胞群中的细胞周期。
Adult stem cells must balance self-renewal and differentiation for tissue homeostasis. The Drosophila ovary has provided a wealth of information about the extrinsic niche signals and intrinsic molecular processes required to ensure appropriate germline stem cell renewal and differentiation. The factors controlling behavior of the more recently identified follicle stem cells of the ovary are less well-understood but equally important for fertility. Here we report that translational regulators play a critical role in controlling these cells. Specifically, the translational regulator Caprin (Capr) is required in the follicle stem cell lineage to ensure maintenance of this stem cell population and proper encapsulation of developing germ cells by follicle stem cell progeny. In addition, reduction of one copy of the gene fmr1, encoding the translational regulator Fragile X Mental Retardation Protein, exacerbates the Capr encapsulation phenotype, suggesting Capr and fmr1 are regulating a common process. Caprin was previously characterized in vertebrates as Cytoplasmic Activation/Proliferation-Associated Protein. Significantly, we find that loss of Caprin alters the dynamics of the cell cycle, and we present evidence that misregulation of CycB contributes to the disruption in behavior of follicle stem cell progeny. Our findings support the idea that translational regulators may provide a conserved mechanism for oversight of developmentally critical cell cycles such as those in stem cell populations.