Serum and skin levels of miR-369-3p in patients with psoriasis and their correlation with disease severity
Serum and skin levels of miR-369-3p in patients with psoriasis and their correlation with disease severity
复制标题
银屑病患者血清和皮肤中miR-369-3p的水平及其与疾病严重程度的相关性
DOI：
10.1684/ejd.2013.2148
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发表时间：
2013-09-01
影响因子：
2.5
通讯作者：
Li, Chunying
中科院分区：
文献类型：
--
作者：
Guo, Sen;Zhang, Weigang;Li, Chunying
背景：银屑病是一种常见的自身免疫性皮肤病，以角质形成细胞的强烈增殖和异常分化为特征。近年来，一些miRNAs已被证实在银屑病中异常表达，并在银屑病的发病机制中发挥作用。目的：检测银屑病患者血清和皮肤miR-369-3p水平，探讨其与银屑病严重程度的关系。并探讨了miR-369-3p与肿瘤坏死因子-α之间的调控机制。方法：利用多重预测软件对miRNAs靶基因进行生物信息学分析。采集血清和皮肤组织标本，测定血清和皮肤miR-369-3p水平。评价患者银屑病面积严重程度指数评分及其与血清和皮肤miR-369-3p水平的相关性。用瞬时转染法和酶联免疫吸附试验检测miR-369-3p在HaCaT细胞中的表达与肿瘤坏死因子-α的关系。结果：银屑病患者血清和皮肤miR-369-3p水平均高于健康对照组(P
Background: Psoriasis is a common autoimmune skin disease, characterized by intense proliferation and abnormal differentiation of keratinocytes. Recently, some miRNAs have been proven to show aberrant expression in psoriasis and play a role in the pathogenesis of the disease. Objectives: The aim of this study was to detect serum and skin miR-369-3p levels in patients with psoriasis and confirm their correlation with disease severity. The regulatory mechanism between miR-369-3p and TNF-alpha was also investigated. Methods: Bioinformatics analysis for target genes of miRNAs was performed using multiple prediction software. Serum samples and skin tissues were collected and serum and skin miR-369-3p levels were measured. The Psoriasis Area Severity Index scores of patients and the correlation with serum and skin miR-369-3p levels were evaluated. Transient transfection and Elisa were applied to HaCaT cells to testify the relationship between expression of miR-369-3p and TNF-alpha. Results: Serum and skin miR-369-3p levels were both higher in patients with psoriasis than those in healthy controls (P