Enhanced biocontrol activity of cellulase from Trichoderma harzianum against Fusarium graminearum through activation of defense-related genes in maize
Enhanced biocontrol activity of cellulase from Trichoderma harzianum against Fusarium graminearum through activation of defense-related genes in maize
复制标题
通过激活玉米中的防御相关基因增强哈茨木霉纤维素酶对禾谷镰刀菌的生物防治活性
DOI：
10.1016/j.pmpp.2018.05.004
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Saravanakumar, Kandasamy;Dou, Kai;Chen, Jie
本文通过体外、植物体内和虚拟分子模拟实验，研究了哈茨木霉（Trichoderma harzianum）Th22菌株的纤维素酶对玉米根中2，4-二羟基-7-甲氧基-2H-1，4-苯并恶嗪-3-（4H）-酮（DIMBOA）的诱导作用及其对玉米根防御相关基因表达的影响。qRT-PCR和HPLC分析结果表明，纤维素酶基因Thph1和2通过激活玉米根中JA、ET、SAR、DIMBOA和PII相关基因的表达参与了FG及其相关植物病害的生防。此外，抑制试验还证实，丁布阿以浓度依赖性方式抑制FG的生长。此外，虚拟研究表明，玉米根中的丁布A代谢或抑制FG中的致病活性位点（FgSwi6）和真菌毒素相关蛋白（FgAtf1）。最后，本研究表明，T.哈茨木霉不仅限于防治叶部病害，还参与镰刀菌茎腐病的生防。
This work reports the role of cellulase from Trichoderma harzianum strain Th22 in stimulation of 2,4-Dihydroxy-7- methoxy-2H-1,4-benzoxazin-3-(4H)-one (DIMBOA) and defense-related gene expressions in maize root against deoxynivalenol producing Fusarium graminearum (FG) by in vitro, in planta and virtual molecular modeling experiments. The results indicated that cellulase genes such as Thph1& 2 were involved in biocontrol of FG and its related plant diseases through the activation of JA, ET, SAR, DIMBOA and PII related gene expressions in the maize root as evidenced by qRT-PCR and HPLC analysis. Further, the inhibitory assay also confirms that DIMBOA inhibited the growth of FG at concentrations dependent manner. Furthermore, virtual studies revealed that DIMBOA from maize root metabolizes or inhibit the active site of pathogenicity (FgSwi6) and mycotoxin (FgAtf1) related protein in FG. Finally, this work concluded that cellulase genes Thph1 and Thph2 from T. harzianum was not only limited to control of foliar disease but also involved in biocontrol of Fusarium stalk rot.