Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2
Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2
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DOI：
10.1128/cvi.00208-07
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发表时间：
2008-03-01
影响因子：
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通讯作者：
Guzman, Maria G.
中科院分区：
文献类型：
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作者：
Bernardo, Lidice;Izquierdo, Alienys;Guzman, Maria G.
本研究的目的是比较登革病毒2型（DENV-2）美洲和亚洲基因型诱导的猕猴免疫反应和保护能力。动物感染美国或亚洲DENV-2毒株，1年后用亚洲DENV-2基因型毒株攻毒。不同毒株原发感染和继发感染后的病毒血症和猴抗体水平相似；然而，抗体反应的功能是不同的。所有猴子继发性感染后，病毒复制有限。在组织培养中没有分离到病毒，而逆转录pcr显示，5只受激猴子中有4只出现了晚期阳性反应。免疫球蛋白M反应模式和免疫印迹法检测特异性蛋白抗体支持保护数据。尽管证明了同源攻击后的保护作用，但观察到强烈的遗忘抗体反应。
The goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (DENV-2) American and Asian genotypes in Macaca fascicularis monkeys. Animals were infected with American or Asian DENV-2 strains and challenged 1 year later with a DENV-2 Asian genotype strain. The viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. A limited viral replication was demonstrated after the secondary infection in all the monkeys. No virus was isolated in tissue culture, while reverse transcription-PCR showed a late positive reaction in four of five challenged monkeys. The immunoglobulin M response pattern and the detection of antibodies to specific proteins by Western blotting supported the protection data. Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed.