CELLULAR IMMUNE-RESPONSE TOWARD HUMAN ARTICULAR CHONDROCYTES - T-CELL REACTIVITIES AGAINST CHONDROCYTE AND FIBROBLAST MEMBRANES IN DESTRUCTIVE JOINT DISEASES
CELLULAR IMMUNE-RESPONSE TOWARD HUMAN ARTICULAR CHONDROCYTES - T-CELL REACTIVITIES AGAINST CHONDROCYTE AND FIBROBLAST MEMBRANES IN DESTRUCTIVE JOINT DISEASES
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DOI：
10.1002/art.1780331004
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发表时间：
1990-10-01
影响因子：
--
通讯作者：
BURMESTER, GR
中科院分区：
文献类型：
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作者：
ALSALAMEH, S;MOLLENHAUER, J;BURMESTER, GR
关节软骨是人类破坏性关节疾病的主要靶点之一。我们研究了针对软骨细胞表面膜的细胞免疫反应，因为最近有人提出，基于对某些关节疾病中具有这种特异性的自身抗体的观察，这些反应代表了可能的抗原结构。在类风湿关节炎患者的血液和滑膜组织中发现了对软骨细胞膜的惊人T细胞反应性。这种反应性强烈依赖于单核细胞的存在，并具有抗原驱动过程的所有特征。克隆分析表明，外周血T细胞前体频率高，对软骨细胞膜有反应。这种对软骨细胞膜的反应大大超过了其他来源如成纤维细胞或上皮细胞的膜诱导的T细胞刺激。与类风湿关节炎患者相比，骨关节炎患者不仅对软骨细胞膜，而且对成纤维细胞膜材料表现出强烈的外周血和滑膜液T细胞反应。但对上皮细胞膜无反应性。正常供体一般对任何膜制剂均无明显反应。这些数据表明，在破坏性关节疾病中存在强烈的T细胞对软骨细胞膜的反应性，这可能在这些疾病中发生的致病过程中起重要作用。
Articular cartilage is one of the major targets in destructive joint diseases in humans. We studied cellular immune reactions against cartilage cell-surface membranes, because it has recently been suggested that these represent possible antigenic structures, based upon the observation of autoantibodies with this specificity in certain joint diseases. A striking T cell reactivity toward chondrocyte membranes was found both in blood and synovial tissue from patients with rheumatoid arthritis. This reactivity was strongly dependent on the presence of monocytes and had all the characteristics of an antigen-driven process. Clonal analysis demonstrated high precursor frequencies in peripheral blood T cells that were reactive against chondrocyte membranes. This response to chondrocyte membranes greatly exceeded the T cell stimulation induced by membranes from other sources such as fibroblasts or epithelial cells. In contrast to patients with rheumatoid arthritis, individuals with osteoarthritis showed a strong peripheral blood and synovial fluid T cell response not only to chondrocyte membranes, but also to fibroblast membrane material. However, there was no reactivity to epithelial cell membranes. Normal donors generally did not show significant responses to any membrane preparation. These data indicate that there is a strong T cell reactivity toward chondrocyte membranes in destructive joint disorders, and this may significantly contribute to the pathogenic processes that occur in these diseases.