Practical approach for typing strains of Leishmania infantum by enzyme polymorphism: a cross sectional study in northwest of Iran.
Practical approach for typing strains of Leishmania infantum by enzyme polymorphism: a cross sectional study in northwest of Iran.
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DOI：
10.3923/pjbs.2007.4505.4509
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发表时间：
2007-12-15
影响因子：
--
通讯作者：
Davies, C.R.
中科院分区：
文献类型：
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作者：
Gavgani, A.S. Mazloumi;Ghazanchaei, A.;Davies, C.R.
在本研究中，所有样本均在 2004 年至 2006 年间从伊朗西北部的 Kalybar 和 Ahar 地区采集，包括 12 名患者（骨髓抽吸物）、26 只狗（脾脏和肝脏抽吸物）以及 100 多只白蛉。所有患者均经临床诊断患有内脏利什曼病。人类和狗的所有血清样本的血清学特征均符合利什曼病 (DAT)。使用 12 种酶系统将这些分离物的同工酶谱与参考同工酶谱进行比较。 L. infuntum MON-1 是唯一存在于狗、沙蝇和人类所有样本中的酶调制解调器。通过与邻国（阿塞拜疆、伊拉克和土耳其等）的酶多态性比较，我们得出结论，伊朗西北部的内脏利什曼病（VL）疫源地显然是人畜共患VL的地中海疫源地，其范围从葡萄牙和摩洛哥延伸到巴基斯坦和中亚各共和国。家养犬是储存宿主，其中坎德拉白蛉（Phlebotomus kandelakii）和佩菲利维阿里亚西（Perfiliewi ariasi）是媒介。
In present study, All samples collected from Kalybar and Ahar districts in Northwest of Iran from 12 patients (bone marrow aspirates), 26 dogs (spleenic and hepatic aspirates) and more than 100 sand flies between years 2004-2006. All patients were clinically diagnosed to have visceral leishmaniasis. Serological profiles of all sera samples from both human and dogs were in accordance with leishmaniasis (DAT). Isoenzyme profiles of these isolates were compared with those of reference using 12 enzyme systems. L. infuntum MON-1 is the only zymodeme present in all samples of dogs, sand flies and human. The enzymatic polymorphism is compared to that of neighboring countries (Azarbaijan, Iraq and Turkey etc.) and we concluded that the Visceral Leishmaniasis (VL) focus in northwest of Iran is evidently Mediterranean focus of zoonotic VL, which extends from Portugal and Morocco to Pakistan and the central Asian republics. Domestic doges act as the reservoir host, where Phlebotomus kandellakii and Perfiliewi ariasi are vectors.