Protein folding in the cell: challenges and progress.
Protein folding in the cell: challenges and progress.
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DOI：
10.1016/j.sbi.2010.11.001
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发表时间：
2011-02
影响因子：
6.8
通讯作者：
Gierasch, Lila M.
中科院分区：
文献类型：
--
作者：
Gershenson, Anne;Gierasch, Lila M.
很难想象比用于体外蛋白质折叠研究的稀溶液与拥挤、分隔、粘稠、空间不均匀的细胞内部之间的对比更极端的对比了。这篇综述重点介绍了最近探索细胞中蛋白质折叠的研究，重点关注通常与体外蛋白质折叠研究无关的问题，例如大分子拥挤、扩散受阻、共翻译折叠、分子伴侣和进化压力。表征细胞中蛋白质折叠所必须克服的技术障碍正在推动方法学的进步，我们提请注意几个例子，例如细胞中折叠的荧光成像和细胞内稳定性的遗传筛选。
It is hard to imagine a more extreme contrast than that between the dilute solutions used for in vitro studies of protein folding and the crowded, compartmentalized, sticky, spatially inhomogeneous interior of a cell. This review highlights recent research exploring protein folding in the cell with a focus on issues that are generally not relevant to in vitro studies of protein folding, such as macromolecular crowding, hindered diffusion, co-translational folding, molecular chaperones, and evolutionary pressures. The technical obstacles that must be overcome to characterize protein folding in the cell are driving methodological advances, and we draw attention to several examples, such as fluorescence imaging of folding in cells and genetic screens for in-cell stability.
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影响因子：
16
作者：
Feige, Matthias J.;Groscurth, Sandra;Marcinowski, Moritz;Shimizu, Yuichiro;Kessler, Horst;Hendershot, Linda M.;Buchner, Johannes
通讯作者：
Buchner, Johannes
影响因子：
2.4
作者：
Counago, Rafael;Wilson, Corey J.;Shamoo, Yousif
通讯作者：
Shamoo, Yousif
影响因子：
64.5
作者：
Chakraborty, Kausik;Chatila, Manal;Hayer-Hartl, Manajit
通讯作者：
Hayer-Hartl, Manajit
影响因子：
2.9
作者：
Christiansen, Alexander;Wang, Qian;Wittung-Stafshede, Pernilla
通讯作者：
Wittung-Stafshede, Pernilla
DOI：
10.1038/nsb1001-879
发表时间：
2001-10-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Ghaemmaghami, S;Oas, TG
通讯作者：
Oas, TG