Identification and Mode of Inheritance of Quantitative Trait Loci for Secondary Metabolite Abundance in Tomato
Identification and Mode of Inheritance of Quantitative Trait Loci for Secondary Metabolite Abundance in Tomato
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DOI：
10.1105/tpc.114.132266
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发表时间：
2015-03-01
期刊：
影响因子：
11.6
通讯作者：
Fernie, Alisdair R.
中科院分区：
文献类型：
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作者：
Alseekh, Saleh;Tohge, Takayuki;Fernie, Alisdair R.
为研究番茄果实果皮次生代谢相关的基因组区域，对特征良好的半夏茄（Solanum pennellii）渗入系进行了大规模代谢数量性状位点（mQTL）分析。总共在76条渗入线上检测到679个mqtl。遗传力分析表明，次生代谢的mqtl受环境的影响小于初级代谢的mqtl。网络分析使我们能够评估初级和次级代谢的相互联系，并比较和对比它们各自与形态特征的关联。此外，我们应用了最近建立的实时定量PCR平台来深入了解mqtl子集的转录控制机制，包括羟基肉桂酸、酰基糖、柚皮素查尔酮和一系列糖生物碱。有趣的是，许多这些化合物表现出显性-负遗传模式，这与传统的观点相反，即次生代谢物含量随着驯化而减少。此外，我们还通过病毒诱导的基因沉默对糖生物碱mQTLs的两个候选基因进行了示范性评估。将本研究的综合数据与前人在相同材料中获得的初级代谢结果和其他次生代谢自然方差研究结果进行比较。
A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgression lines. Heritability analyses revealed that mQTLs of secondary metabolism were less affected by environment than mQTLs of primary metabolism. Network analysis allowed us to assess the interconnectivity of primary and secondary metabolism as well as to compare and contrast their respective associations with morphological traits. Additionally, we applied a recently established real-time quantitative PCR platform to gain insight into transcriptional control mechanisms of a subset of the mQTLs, including those for hydroxycinnamates, acyl-sugar, naringenin chalcone, and a range of glycoalkaloids. Intriguingly, many of these compounds displayed a dominant-negative mode of inheritance, which is contrary to the conventional wisdom that secondary metabolite contents decreased on domestication. We additionally performed an exemplary evaluation of two candidate genes for glycolalkaloid mQTLs via the use of virus-induced gene silencing. The combined data of this study were compared with previous results on primary metabolism obtained from the same material and to other studies of natural variance of secondary metabolism.