Biological properties of lithium-containing surface pre-reacted glass fillers as direct pulp-capping cements
Biological properties of lithium-containing surface pre-reacted glass fillers as direct pulp-capping cements
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DOI：
10.1016/j.dental.2021.12.011
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发表时间：
2022-02-05
期刊：
影响因子：
5
通讯作者：
Hayashi，Mikako
中科院分区：
文献类型：
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作者：
Ali，Manahil;Okamoto，Motoki;Hayashi，Mikako
表面预反应玻璃填料（S-PRG）可以释放不同类型的离子，在我们以前的研究中，我们用氯化锂（S-PRG/Li-100 mM）修饰这些填料，通过激活Wnt/β-catenin信号通路诱导修复性牙本质形成。在这里，我们评估了S-PRG/Li-100 mM的生物学性能，并将其与三氧化矿物质聚集体（MTA）和S-PRG无additives.MethodsIn体内研究进行了对雄性Wistar大鼠使用马森三色染色在髓盖磨牙。将试验材料皮下植入，以评价其血管化能力和生物相容性。通过将测试材料浸入模拟体液中来测试它们形成磷灰石的能力。结果经Masson三色染色和组织植入试验证实，S-PRG/Li-100 mM具有良好的生物相容性，与MTA和S-PRG相似;术后14天皮下组织炎症消退。S-PRG/Li-100 mM促进其表面磷灰石的形成。S-PRG组对口腔细菌生物膜的封闭能力和杀菌/抑菌活性均高于MTA。显著性含锂表面预反应玻璃水门汀的抗菌和封闭能力优于MTA，表明其作为高性能直接盖髓材料的潜力。
ObjectiveSurface pre-reacted glass fillers (S-PRG) can release different types of ions and in our previous study, we modified these fillers with lithium chloride (S-PRG/Li-100 mM) to induce reparative dentin formation by activating the Wnt/β-catenin signaling pathway. Here, we assessed the biological performance of S-PRG/Li-100 mM and compared it with that of mineral trioxide aggregate (MTA) and S-PRG without additives.MethodsIn vivostudies were conducted on male Wistar rats using Masson’s trichrome staining in pulp-capped molars. The test materials were implanted subcutaneously to evaluate their capacity for vascularization and biocompatibility. The ability of the test materials to form apatite was tested by immersing them in simulated body fluid. Rhodamine-B staining was conducted to assess their sealing ability in bovine teeth, while their antibacterial activity was evaluated againstStreptococcus mutansandLactobacillus caseiin terms of colony-forming units and by live/dead staining.ResultsMasson’s trichrome staining and tissue-implantation tests confirmed the biocompatibility of S-PRG/Li-100 mM and it was similar to that of MTA and S-PRG; inflammation regression was observed 14 days after operation in the subcutaneous tissues. S-PRG/Li-100 mM promoted the formation of apatite on its surface. Both the S-PRG groups showed higher sealing capability and bactericidal/bacteriostatic activity against oral bacterial biofilms than MTA.SignificanceLithium-containing surface pre-reacted glass cements exhibit better antibacterial and sealing capabilities than MTA, suggesting their potential as high-performance direct pulp-capping materials.