A FAST TRANSIENT HYPERPOLARIZATION OCCURS DURING THE SEA-URCHIN SPERM ACROSOME REACTION INDUCED BY EGG JELLY
A FAST TRANSIENT HYPERPOLARIZATION OCCURS DURING THE SEA-URCHIN SPERM ACROSOME REACTION INDUCED BY EGG JELLY
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DOI：
10.1016/0014-5793(87)81055-4
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发表时间：
1987-06-29
期刊：
影响因子：
3.5
通讯作者：
DARSZON, A
中科院分区：
文献类型：
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作者：
GONZALEZMARTINEZ, M;DARSZON, A
用膜电位敏感染料diS-C3-(5)测定了蛋胶诱导的海胆精子去极化，在此之前，快速猝灭被认为是一种人工产物。在这里，我们发现部分猝灭是由K+相关的瞬态超极化（~ 4 s）引起的。含有25-35 mM KCl的海水可抑制果冻或从中纯化的顶体反应诱导因子诱导的超极化、去极化和顶体反应。这些结果表明，蛋冻诱导瞬时超极化是由K+通透性增加介导的，这可能参与了顶体反应的触发
The egg jelly-induced sea urchin sperm depolarization, assayed with the membrane potential-sensitive dye diS-C3-(5), is preceded by a fast quenching, which was initially taken as an artifact. Here we show that part of this quenching results from a K+-dependent transient hyperpolarization (∼4 s). Seawater containing 25–35 mM KCl inhibits this hyperpolarization, the depolarization and the acrosome reaction induced by egg jelly, or by the acrosome reaction inducing factor purified therefrom. These results suggest that egg jelly induces a transient hyperpolarization mediated by an increase in K+permeability, which may be involved in triggering the acrosome reaction