Expression profiling identifies a molecular signature of reactive astrocytes stimulated by cyclic AMP or proinflammatory cytokines
Expression profiling identifies a molecular signature of reactive astrocytes stimulated by cyclic AMP or proinflammatory cytokines
复制标题
DOI：
10.1016/j.expneurol.2007.10.016
复制
发表时间：
2008-03-01
影响因子：
5.3
通讯作者：
Gutmann, David H.
中科院分区：
文献类型：
--
作者：
Daginakatte, Girish C.;Gadzinski, Adam;Gutmann, David H.
特化胶质细胞，称为反应性星形胶质细胞，伴随着许多影响中枢神经系统的病理条件，包括中风，多发性硬化和肿瘤。为了更好地定义这种重要的细胞类型，我们采用了高密度微阵列基因表达谱，使用两种体外反应性神经胶质增生模型（用dbcAMP或IL-1 β/IFN-γ刺激）。我们确定了44个差异表达的转录共同的两个体外模型，并证明了这些基因的一个子集也差异表达的实验性自身免疫性脑脊髓炎和局灶性脑缺血在体内。此外，在过度增殖或肿瘤性胶质细胞中未观察到这种差异基因表达模式。(C)2007年爱思唯尔公司All rights reserved.
Specialized glia, termed reactive astrocytes, accompany numerous pathologic conditions affecting the central nervous system, including stroke, multiple sclerosis, and neoplasia. To better define this important cell type, we employed high-density microarray gene expression profiling using two in vitro models of reactive gliosis (stimulation with dbcAMP or IL-1 beta/IFN-gamma). We identified 44 differentially expressed transcripts common to both in vitro models and demonstrated that a subset of these genes are also differentially expressed in response to experimental autoimmune encephalomyelitis and focal cerebral ischemia in vivo. Moreover, this pattern of differential gene expression is not observed in hyperproliferating or neoplastic glia. (C) 2007 Elsevier Inc. All rights reserved.