ELISA for the detection of antibodies to Ebola viruses
ELISA for the detection of antibodies to Ebola viruses
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DOI：
10.1086/514313
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发表时间：
1999-02-01
影响因子：
6.4
通讯作者：
Peters, CJ
中科院分区：
文献类型：
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作者：
Ksiazek, TG;West, CP;Peters, CJ
针对埃博拉 (EBO) 病毒抗原的 Ige 和 IgM 抗体的 EIA 已使用感染 EBO 病毒后幸存的动物和人类血清进行开发和评估。 IgM 捕获测定在 5 只实验感染动物中的 5 只死于致命感染时，其血清中检测到了抗 EBO（雷斯顿亚型）抗体。在对感染 EBO（雷斯顿亚型）的猴子进行尸检时，其血清中也检测到了 IgM 抗体。该抗体早在实验感染动物感染后第 6 天就可检测到，并在 3 只感染后幸存的动物中持续存在 400 天，在 2 名人类的血清中，该抗体在感染后持续存在约 10 年。尽管这些数据受到可用于验证的血清数量的限制，但 IgM 检测似乎作为一种诊断工具具有很大的前景。此外，通过该测试测量的 IgG 抗体的长期持续性强烈表明 ELISA 将可用于 EBO 病毒的现场研究。
EIAs for Ige and IgM antibodies directed against Ebola (EBO) viral antigens have been developed and evaluated using sera of animals and humans surviving infection with EBO viruses. The IgM capture assay detected anti-EBO (subtype Reston) antibodies in the sera of 5 of 5 experimentally infected animals at the time they succumbed to lethal infections. IgM antibodies were also detected in the serum of a human who was infected with EBO (subtype Reston) during a postmortem examination of an infected monkey. The antibody was detectable as early as day 6 after infection in experimentally infected animals and persisted for 400 days in 3 animals who survived infection, and it persisted for similar to 10 years after infection in the sera of 2 humans. Although these data are limited by the number of sera available for verification, the IgM assay seems to have great promise as a diagnostic tool. Furthermore the long-term persistence of the IgG antibodies measured by this test strongly suggests that the ELISA will be useful in field investigations of EBO virus.