Characterization of Agrobacterium tumefaciens strains isolated from grapevine tumors in China.
Characterization of Agrobacterium tumefaciens strains isolated from grapevine tumors in China.
复制标题
从中国葡萄肿瘤中分离的根癌农杆菌菌株的鉴定。
DOI：
10.1128/aem.53.6.1338-1343.1987
复制
发表时间：
1987
影响因子：
4.4
通讯作者：
Nester,EW
中科院分区：
文献类型：
--
作者：
Ma,DQ;Yanofsky,MF;Gordon,MP;Nester,EW
对中国北方葡萄肿瘤中分离的13株农杆菌进行了调查。这些菌株在其宿主范围特性上各不相同，尽管它们都对葡萄藤具有致瘤性。其中12株为农杆菌3型，11株在肿瘤组织中合成章鱼碱。有趣的是，一种菌株导致精氨酸在肿瘤组织中积累，精氨酸是一种以前未被识别的嘌呤。虽然这些毒株中的大多数DNA与先前表征的转移DNA和vir位点同源，但一些毒力毒株与这些位点很少或没有同源性。因此，这些菌株中的一些代表了农杆菌的广泛不同的例子。大多数菌株的DNA与广泛宿主范围的病毒位点几乎没有同源性，但与有限宿主范围的病毒位点具有很强的同源性。这一发现进一步支持了与葡萄藤相关的农杆菌菌株可能具有特定病毒位点的观点。
Thirteen strains of Agrobacterium tumefaciens isolated from grapevine tumors in northern China were surveyed. These strains varied in their host range properties, although all were tumorigenic on grapevines. Twelve of these strains belonged to Agrobacterium sp. biotype 3, and 11 strains resulted in the synthesis of the opine octopine in tumor tissue. Interestingly, one strain resulted in accumulation of arginine, a previously unrecognized opine, in tumor tissue. Although DNA in most of these strains showed homology to the previously characterized transferred DNA and vir loci, some virulent strains showed little or no homology to these loci. Thus, some of these strains represent widely divergent examples of Agrobacterium sp. The DNA in most strains exhibited little or no homology to a wide-host-range virA locus but did show strong homology to a limited-host-range virA locus. This finding further supports the idea that Agrobacterium strains associated with grapevines may have a specific virA locus.