A cost-effective cane molasses medium for enhanced cell-bound phytase production by Pichia anomala
A cost-effective cane molasses medium for enhanced cell-bound phytase production by Pichia anomala
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DOI：
10.1111/j.1365-2672.2004.02327.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Satyanarayana, T
中科院分区：
文献类型：
--
作者：
Vohra, A;Satyanarayana, T
目的：配制一种廉价的甘蔗糖蜜培养基，以提高异常毕赤酵母的细胞结合植酸酶产量。方法和结果：在合成葡萄糖-牛肉提取物和甘蔗糖蜜培养基中比较异常毕赤酵母的细胞结合植酸酶产量。将酵母培养在含有50ml培养基的250ml烧瓶中，接种12小时的接种物(3x10(6)CFUml(-1))并在25℃以250转min(-1)培养24小时。在分批发酵的甘蔗糖蜜培养基中优化了不同的培养参数。与合成培养基(100 U g(-1)干生物量)相比，甘蔗糖蜜培养基(176 U g(-1)干生物量)中细胞结合植酸酶含量显着增加。在补料分批发酵中，甘蔗糖蜜培养基中的生物量（20 g l(-1)）和植酸酶产量（3000 U l(-1)）显着增加。甘蔗糖蜜培养基的生产成本为每 1000 U 英镑 0.006 英镑，与合成培养基（每 1000 U 0.25 英镑）相比要低得多。结论：与合成培养基相比，采用补料分批发酵的优化甘蔗糖蜜培养基的植酸酶产量总体提高了 86.6%。此外，在甘蔗糖蜜培养基中生产具有成本效益。 研究意义和影响：与合成培养基相比，甘蔗糖蜜中植酸酶的产量得到提高，并且生产成本也显着降低。这种酶可以在动物饲料工业中得到应用，以改善饲料的营养状况和对抗环境污染。
Aim: Formulation of an inexpensive cane molasses medium for improved cell-bound phytase production by Pichia anomala.Methods and Results: Cell-bound phytase production by Pichia anomala was compared in synthetic glucose-beef extract and cane molasses media. The yeast was cultivated in 250 ml flasks containing 50 ml of the medium, inoculated with a 12 h-old inoculum (3x10(6) CFU ml(-1)) and incubated at 25degreesC for 24 h at 250 rev min(-1). Different cultural parameters were optimized in cane molasses medium in batch fermentation. The cell-bound phytase content increased significantly in cane molasses medium (176 U g(-1) dry biomass) when compared with the synthetic medium (100 U g(-1) dry biomass). In fed-batch fermentation, a marked increase in biomass (20 g l(-1)) and the phytase yield (3000 U l(-1)) were recorded in cane molasses medium. The cost of production in cane molasses medium was pound0.006 per 1000 U, which is much lower when compared with that in synthetic medium (pound0.25 per 1000 U).Conclusions: An overall 86.6% enhancement in phytase yield was attained in optimized cane molasses medium using fed-batch fermentation when compared with that in synthetic medium. Furthermore, the production in cane molasses medium is cost-effective.Significance and Impact of the Study: Phytase yield was improved in cane molasses when compared with the synthetic medium, and the cost of production was also significantly reduced. This enzyme can find application in the animal feed industry for improving the nutritional status of feed and combating environmental pollution.