Dual-wavelength ratiometric immunosensor for Bacillus cereus: Oxidase-like MnO2-Au trigged “OFF-ON” detection strategy
Dual-wavelength ratiometric immunosensor for Bacillus cereus: Oxidase-like MnO2-Au trigged “OFF-ON” detection strategy
复制标题
用于蜡样芽孢杆菌的双波长比率免疫传感器：类氧化酶 MnO2-Au 触发 - 关闭 - 开启 - 检测策略
DOI：
10.1016/j.snb.2022.131925
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Liu Yingju
中科院分区：
文献类型：
--
作者：
Meng Jingnan;Shen Haoran;Luo Lin;Wang Juan;Xu Zhen-Lin;Liu Yingju
As an opportunistic pathogen,Bacillus cereus(B. cereus) has caused increasing emetic and diarrheal food poisoning, even fulminant hepatic failure and death case. Herein, a mimetic oxidase-mediated ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) was developed to detectB. cereus.In this system, 3D MnO2-Au nanoflowers with layered crystalline structure can oxidizeo-phenylenediamine (OPD) to generate a fluorescence peak at 570 nm (I570, response signal), while blue carbon quantum dots (bCDs) display a fluorescence peak at 455 nm (I455, reference signal). Under the effect of fluorescence resonance energy transfer (FRET), the response signal increased while the reference signal was weakened. By using MnO2-Au as the mimetic oxidase to replace the horseradish peroxidase and associate with antibody in traditional ELISA, a linear relationship was established between the ratiometric fluorescence signal (I570/I455) and the logarithm ofB. cereusconcentrations in the range from 1.7 × 102to 3.3 × 106CFU/mL. Comparing with conventional sandwich ELISA, the detection time was shortened by 43 min and the sensitivity was improved by 5-folds, indicating that the proposed ratiometric immunoassay holds great potential as a rapid and convenient tool for sensitive screening ofB. cereus.
