Identification and expression profiling of the Aux/IAA gene family in Chinese hickory (Carya cathayensis Sarg.) during the grafting process
Identification and expression profiling of the Aux/IAA gene family in Chinese hickory (Carya cathayensis Sarg.) during the grafting process
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中国山核桃嫁接过程中 Aux/IAA 基因家族的鉴定及表达谱
DOI：
10.1016/j.plaphy.2018.03.010
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发表时间：
2018
影响因子：
6.5
通讯作者：
Bingsong Zheng
中科院分区：
文献类型：
--
作者：
Huwei Yuan;Liang Zhao;Juanjuan Chen;Ying Yang;Dongbin Xu;Shenchen Tao;Shan Zheng;Yirui Shen;Yi He;Chenjia Shen;Daoliang Yan;Bingsong Zheng
生长素是生物体生长发育的重要调节因子。Aux/IA基因家族的成员编码短寿命的核蛋白，并介导生长素调节的基因表达的反应。本研究首次对中国山核桃Aux/IAA家族的22个开放阅读框架cDNA（命名为CcIAA）进行了鉴定和鉴定。这些基因编码的蛋白质含有Aux/IAA家族的四个完整或部分保守的结构域。系统发育分析表明，CcIAAs在8个不同的亚群中分布不均匀。空间特异性表达谱显示，大部分CcIAAspRNA在特定组织中表达。CcIAA 11、CcIAA 27 a2和CcIAAx基因主要在茎中表达。CcIAAs基因在茎中的优势表达可能在移植过程中血管重连中起重要作用。并对Aux/IAAs基因在山核桃嫁接过程中的表达谱进行了分析。结果表明，在嫁接过程中，有19个CcIAAs表达下调，3个CcIAAs（包括CcIAA 28、CcIAA 8a和CcIAA 27 b）被诱导表达，表明它们在嫁接过程中发生了特异性表达。CcIAA基因在嫁接后早期的参与使我们有机会了解生长素信号在嫁接过程中的作用。
Auxin is an essential regulator in various aspects of organism growth and development. Members of theAux/IAAfamily of genes encode short-lived nuclear proteins and mediate the responses of auxin-regulated gene expression. Here, the first identification and characterization of 22 cDNAs encoding the open reading frame of theAux/IAAfamily in Chinese hickory (named asCcIAA) has been performed. The proteins encoded by these genes contain four whole or partially conserved domains of the Aux/IAA family. Phylogenetic analysis indicated that CcIAAs were unevenly distributed among eight different subgroups. The spatio-specific expression profiles showed that most of theCcIAAspreferentially expressed in specific tissues. ThreeCcIAAgenes, includingCcIAA11,CcIAA27a2andCcIAAx, were predominantly expressed in stem. The predominant expression ofCcIAAgenes in stems might play important roles in vascular reconnection during the graft process. Furthermore, expression profiles ofAux/IAAgenes during the grafting process of Chinese hickory have been analysed. Our data suggested that 19CcIAAswere down-regulated and 3CcIAAs(includingCcIAA28,CcIAA8aandCcIAA27b) were induced, indicating their specializations during the grafting process. The involvement ofCcIAAgenes at the early stage after grafting gives us an opportunity to understand the role of auxin signalling in the grafting process.