A selective medium to isolate airborne spores of Microdochium nivale, causing winter wheat scab
A selective medium to isolate airborne spores of Microdochium nivale, causing winter wheat scab
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一种分离空气中导致冬小麦赤霉病的雪霉微地孢孢子的选择性培养基
DOI：
10.1007/s10658-013-0324-2
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发表时间：
2014
影响因子：
1.8
通讯作者：
Masanori Koike
中科院分区：
文献类型：
--
作者：
Y. Hayashi;T. Kozawa;D. Aiuchi;M. Tani;Masanori Koike
日本北海道冬小麦赤霉病主要由赤霉病菌（Gibberella zeae）和雪腐微囊菌（Microdochium nivale）引起，可导致显著的产量损失。筛选出了一种适合于G.玉米是以前开发的，但不是为M。雪。因此，本研究的目的是开发一种选择性培养基，用于分离空气中的M。雪。基于Komada的镰刀菌选择培养基的基本组成，对碳源和氮源以及最适合于基本组成的维生素B组分进行了检查。菌丝生长;用乳糖代替半乳糖并与L-天冬酰胺结合可促进雪腐镰刀菌的形成，而以盐酸硫胺素为维生素B源则可促进气生菌丝的形成。在抑菌成分中，螺环菌胺对其他丝状真菌的菌落形成有明显的抑制作用。甲基托布津，M.雪腐镰刀菌表现出抗性，选择性地抑制镰刀菌的生长。只.随后在麦田中使用选择性培养基进行孢子捕获。在被其他丝状真菌污染的情况下，雪腐菌在选择培养基上形成特征性的粉红色菌落，使分化变得容易。总体而言，研究结果表明，本研究开发的LATTS培养基可有效分离空气中的M.雪。
Winter wheat scab in Hokkaido, Japan is caused predominantly byGibberella zeaeandMicrodochium nivaleand can result in significant yield losses. A selective medium for isolation ofG. zeaewas previously developed, but not forM. nivale. The purpose of this study therefore was to develop a selective medium for isolation of airborne spores ofM. nivale. Based on the basic composition of Komada’s Fusarium-selective medium, carbon and nitrogen sources and the most suitable vitamin B component for the basal composition were examined. Hyphal growth ofM. nivalewas promoted when galactose was replaced with lactose and combined with L-asparagine, while aerial hyphal formation increased with thiamine hydrochloride as the vitamin B source. In antimicrobial composition, colony formation of other filamentous fungi was greatly inhibited by spiroxamine. Thiophanate methyl, to whichM. nivaleshows resistance, selectively inhibited the growth ofFusariumspp. only. Spore trapping using the selective medium was subsequently performed in a wheat field.M. nivaleformed characteristic pinkish colonies on the selective medium in the case of contamination with other filamentous fungi, making differentiation easy. Overall, the findings show that LATTS medium developed in this study is effective for isolation of airborne spores ofM. nivale.