Sensitive and specific new enzyme-linked immunosorbent assay for N-ERC/Mesothelin increases its potential as a useful serum tumor marker for mesothelioma
Sensitive and specific new enzyme-linked immunosorbent assay for N-ERC/Mesothelin increases its potential as a useful serum tumor marker for mesothelioma
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DOI：
10.1158/1078-0432.ccr-07-1613
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发表时间：
2008-03-01
影响因子：
11.5
通讯作者：
Hino, Okio
中科院分区：
文献类型：
--
作者：
Shiomi, Kazu;Hagiwara, Yoshiaki;Hino, Okio
背景资料：由于间皮瘤最初进展于胸膜和腹膜表面而不形成肿块，因此很难在早期诊断。这将是非常有用的，以确定一个肿瘤标志物，可用于筛选，使更多的诊断，在早期，可治疗的stage.Materials和方法：我们以前确定N-ERC/间皮素作为一个潜在的生物标志物间皮瘤。在目前的工作中，我们使用了一种新开发的ELISA系统来获得各种临床环境中N-ERC/间皮素水平的数据。共招募了102名健康志愿者。此外，39名患者被诊断为间皮瘤，53名患者被诊断为应与间皮瘤区分的疾病，201名受试者被诊断为石棉相关的非恶性疾病（包括单纯石棉肺暴露）在任何一家合作医院接受过治疗。通过新的ELISA系统测量的血清N-ERC/间皮素水平显示，与从其他患者获得的水平相比，间皮瘤患者的中值极高。组织学类型分析显示，血清N-ERC/mesothelin水平在上皮样型间皮瘤中升高，尤其是上皮样型间皮瘤。结论：N-ERC/mesothelin在间皮瘤尤其是上皮样间皮瘤的诊断中具有良好的应用前景。
Background: Because mesothelioma initially progresses on the surface of the pleura and peritoneum without forming masses, it has been difficult to diagnose at an early stage. It would be very useful to identify a tumor marker that could be used for screening to enable more diagnoses to be made at an early, treatable stage.Materials and Methods: We had previously identified N-ERC/mesothelin as a potential biomarker for mesothelioma. In the current work, we used a newly developed ELISA system to gain data on N-ERC/mesothelin levels in various clinical settings. A total of 102 healthy volunteers were recruited. In addition, 39 patients were diagnosed with mesothelioma, 53 patients were diagnosed with diseases that should be distinguished from mesothelioma, and 201 subjects were diagnosed with asbestos-related nonmalignant diseases (including simple exposure to asbestosis) who were treated at any of the cooperating hospitals were enrolled.Results: Serum N-ERC/mesothelin levels measured by a new ELISA system showed that the median values from patients with mesothelioma were extremely high compared with levels obtained from other patients. Analysis in terms of histologic type showed that serum levels of N-ERC/mesothelin were elevated in epithelioid type mesothelioma, especially. In four important models of clinical settings, the sensitivity and specificity of N-ERC/mesothelin were about 71% to 90% and 88% to 93%, respectively.Conclusion: N-ERC/mesothelin is a very promising tumor marker for mesothelioma, especially epithelioid mesothelioma.