Genetic background determines metabolic phenotypes in the mouse
Genetic background determines metabolic phenotypes in the mouse
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DOI：
10.1007/s00335-008-9107-z
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发表时间：
2008-05-01
期刊：
影响因子：
2.5
通讯作者：
Auwerx, Johan
中科院分区：
文献类型：
--
作者：
Champy, Marie-France;Selloum, Mohammed;Auwerx, Johan
为了评估遗传背景对表型变异的贡献，我们比较了四种近交小鼠品系（C57BL/6J、129SvPas、C3HeB/FeJ 和 Balb/cByJ）在不同年龄的大量生化和代谢参数。我们的结果表明，这些不同的近交系之间存在重要的代谢、血液学和生化差异。大多数这些差异与性别无关，并且在一生中都会维持或加剧。因此，在表型研究中必须仔细定义遗传背景。我们的结果还表明，某些背景更适合研究特定的生理现象，因为不同的小鼠品系具有不同的发展特定生化、血液学和代谢异常的倾向。此外，还可以利用这些遗传差异来识别导致表型异常的新基因/蛋白质。生成和分析基因工程突变小鼠的遗传背景的选择很重要，因为它与环境因素一起是导致表型结果变异的最重要因素之一。
To evaluate the contribution of genetic background to phenotypic variation, we compared a large range of biochemical and metabolic parameters at different ages of four inbred mice strains, C57BL/6J, 129SvPas, C3HeB/FeJ, and Balb/cByJ. Our results demonstrate that important metabolic, hematologic, and biochemical differences exist between these different inbred strains. Most of these differences are gender independent and are maintained or accentuated throughout life. It is therefore imperative that the genetic background is carefully defined in phenotypic studies. Our results also argue that certain backgrounds are more suited to study a given physiologic phenomenon, as distinct mouse strains have a different propensity to develop particular biochemical, hematologic, and metabolic abnormalities. These genetic differences can furthermore be exploited to identify new genes/proteins that contribute to phenotypic abnormalities. The choice of the genetic background in which to generate and analyze genetically engineered mutant mice is important as it is, together with environmental factors, one of the most important contributors to the variability of phenotypic results.