Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue
Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue
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DOI：
10.1111/j.1748-1716.2010.02159.x
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发表时间：
2010-12-01
影响因子：
6.3
通讯作者：
Ohno, H.
中科院分区：
文献类型：
--
作者：
Sakurai, T.;Endo, S.;Ohno, H.
目的：先前的研究表明，与久坐不动的对照组相比，运动训练减少了白色脂肪组织（WAT）的质量，并且较小的质量包含较少的脂肪细胞。然而，运动训练对脂肪形成的影响并不完全清楚。因此，我们重新检查的影响，运动训练对脂肪细胞的数量在WAT，如果这样的效果被发现测试的脂肪形成反应的间质血管部分（SVF）细胞含有脂肪组织来源的干细胞（ADSC）在附睾WAT从exercise-trained（TR）rats.Methods：Wistar雄性大鼠分为两组：对照组（C）和TR。TR大鼠在跑步机上进行9周的运动。通过离心从附睾WAT分离含有ADSC的SVF细胞。结果：在TR大鼠SVF细胞中，过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor gamma，PPAR gamma）及其靶基因的表达明显下调，而前脂肪细胞因子1（preadipocyte factor-1，PF 1）基因的表达明显上调。与C组大鼠相比，TR组大鼠SVF细胞在诱导脂肪细胞分化后的脂质积累明显受到抑制。此外，缺氧诱导因子-1 α（HIF-1 α）蛋白的表达增加，观察到在SVF细胞的TR大鼠。YC-1，一种有效的HIF-1 α抑制剂，在TR大鼠的SVF细胞的预处理恢复adipogenesis.Conclusion：这些结果表明，运动训练抑制SVF细胞分化为脂肪细胞的能力，和潜在的机制涉及HIF-1 α的表达上调。
Aim: Previous studies have shown that exercise training reduced white adipose tissue (WAT) mass compared to that in sedentary controls, and that the smaller mass contained fewer adipocytes. However, the effect of exercise training on adipogenesis is not completely clear. Therefore, we re-examined the effect of exercise training on adipocyte numbers in WAT and, if such an effect was found tested the adipogenic responses of stromal-vascular fraction (SVF) cells containing adipose tissue-derived stem cells (ADSC) in epididymal WAT from exercise-trained (TR) rats.Methods: Wistar male rats were divided into two groups: control (C) and TR. The TR rats were subjected to exercise on a treadmill for 9 weeks. SVF cells containing ADSC were separated from epididymal WAT by centrifugation. Expression of adipocyte differentiation-related genes and adipogenesis of SVF cells were examined.Results: In SVF cells of TR rats, the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) and that of PPAR gamma target lipogenic genes was dramatically downregulated, whereas that of preadipocyte factor-1 gene was significantly upregulated. Lipid accumulation in SVF cells of TR rats after the induction of adipocyte differentiation was significantly suppressed in comparison with that of C rats. Moreover, increased expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) protein was observed in SVF cells of TR rats. Pre-treatment of YC-1, a potent HIF-1 alpha inhibitor, in SVF cells of TR rats restored adipogenesis.Conclusion: These results suggest that exercise training suppresses the ability of SVF cells to differentiate into adipocytes, and that underlying mechanisms involve the upregulation of HIF-1 alpha expression.