Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura
Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura
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DOI：
10.1111/1744-7917.12961
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发表时间：
2021-10-20
期刊：
影响因子：
4
通讯作者：
Lu, Yuzhen
中科院分区：
文献类型：
--
作者：
Li, Qilin;Li, Mengge;Lu, Yuzhen
钙粘蛋白、氨肽酶N（APN）和碱性磷酸酶（ALP）已被表征为Cry受体。本研究对7种昆虫的3个受体家族进行了比较基因组学分析。在系统发育树中，ALP和APN分别被分为3个和8个分支。进化枝3中的ALP和进化枝1中的APN在每个物种内包含多个旁系同源物，并且大多数旁系同源物紧密地位于染色体中。果蝇APNs在进化枝5中扩增，在中肠中低表达。钙粘蛋白可分为16个分支，除了BtR和Cad 89 D类分支外，它们可能在全变态昆虫物种形成之前分化。来自不同目的8种含有BtR直系同源物的昆虫对Cry 1A或Cry 3A敏感，而5种不含BtR的昆虫对这两种毒素不敏感。在斜纹夜蛾等6种昆虫中，第1分支的APNs、第3分支的几个ALP、BtR和Cad 89 D-like基因在幼虫中肠中高度或中度表达，其中几个成员被鉴定为Cry受体。假定的S.还分析了暴露于Bt毒素后中肠中的斜纹夜蛾Cry受体。
Cadherin, aminopeptidase N (APN) and alkaline phosphatase (ALP) have been characterized as Cry receptors. In this study, comparative genomic analysis of the 3 receptor families was performed in 7 insects. ALPs and APNs are divided into three and eight clades in phylogenetic trees, respectively. ALPs in clade 3 and APNs in clade 1 contain multiple paralogs within each species and most paralogs are located closely in chromosomes. Drosophila melanogaster has expanded APNs in clade 5 and were lowly expressed in midgut. Cadherins are divided into 16 clades; they may diverge before holometabolous insect speciation except for BtR and Cad89D-like clades. Eight insects from different orders containing BtR orthologs are sensitive to Cry1A or Cry3A, while five species without BtR are insensitive to both toxins. Most APNs in clade 1, several ALPs in clade 3, BtR and Cad89D-like genes were highly or moderately expressed in larval midgut of Spodoptera litura and the other six species, and several members in these clades have been identified as Cry receptors. Expressions of putative S. litura Cry receptors in the midgut after exposing to Bt toxins were also analyzed.