Diagnosis of pemphigus by ELISA: a critical evaluation of two ELISAs for the detection of antibodies to the major pemphigus antigens, desmoglein 1 and 3
Diagnosis of pemphigus by ELISA: a critical evaluation of two ELISAs for the detection of antibodies to the major pemphigus antigens, desmoglein 1 and 3
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DOI：
10.1046/j.1365-2230.2000.00624.x
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发表时间：
2000-05-01
影响因子：
4.1
通讯作者：
Black, MM
中科院分区：
文献类型：
--
作者：
Harman, KE;Gratian, MJ;Black, MM
寻常型天疱疮（PV）和落叶型天疱疮（PF）的特征在于分别针对桥粒糖蛋白桥粒芯糖蛋白3（Dsg 3）和Dsg 1（Dsg 1）的自身抗体。在这项研究中，两个酶联免疫吸附试验（ELISA）检测IgG自身抗体Dsg 1和Dsg 3已被评估。共有317名正常人和疾病对照，82名PV患者和25名PF患者进行了研究。Dsg 3 ELISA在34例未经治疗的PV患者中均为阳性，Dsg 1 ELISA在10例未经治疗的PF患者中均为阳性。当包括正在接受治疗的患者时，敏感性分别下降至95%和92%，但仍优于间接免疫荧光法的敏感性（PV为79%，Dsg 3 ELISA检测PF血清均为阴性，特异性均在98%以上。可以在相对较短的时间内同时分析大量样品。Dsg 1和Dsg 3 ELISA还提供了客观、定量、可重复的数据，这些数据可以区分PV和PF，鉴于这些优点，它们很可能成为诊断实验室的常规技术。
Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are characterized by autoantibodies to the desmosomal glycoproteins desmoglein 3 (Dsg 3) and Dsg 1 (Dsg 1), respectively. In this study, two enzyme-linked immunosorbent assays (ELISA) which detect IgG autoantibodies to Dsg 1 and Dsg 3 have been evaluated. A total of 317 normal and disease controls, 82 patients with PV and 25 with PF were studied. The Dsg 3 ELISA was positive in all 34 patients with untreated PV and the Dsg 1 ELISA was positive in all 10 with untreated PF. When patients undergoing treatment were included, the sensitivities fell to 95% and 92%, respectively, but still compared favourably to the sensitivity of indirect immunofluorescence which was 79% in PV and 84% in PF. All PF sera were negative in the Dsg 3 ELISA and the specificity of both assays was 98% or greater. Large numbers of samples could be analysed simultaneously over a relatively short time period. The Dsg 1 and Dsg 3 ELISAs also provided objective, quantitative, reproducible data which allowed differentiation of PV from PF and in view of these advantages, they are likely to become a routine technique in diagnostic laboratories.