Preferential expression of the novel alternative isoform I.3 of hypoxia-inducible factor lot in activated human T lymphocytes
Preferential expression of the novel alternative isoform I.3 of hypoxia-inducible factor lot in activated human T lymphocytes
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DOI：
10.1016/j.humimm.2008.05.004
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发表时间：
2008-07-01
期刊：
影响因子：
2.7
通讯作者：
Sitkovsky, Michail
中科院分区：
文献类型：
--
作者：
Lukashev, Dmitriy;Sitkovsky, Michail
缺氧诱导因子 1 α (HIF-1 α) 不仅在氧稳态的调节中至关重要，而且在先天性和适应性免疫系统的调节中也至关重要。我们之前报道过，小鼠中 T 细胞受体介导的 T 细胞激活导致了 a 的表达。 HIF-1 α 的另一种亚型，以延迟负反馈方式抑制激活的 T 细胞。在本文中，我们描述了人类 HIF-1 α 的一种新型 mRNA 亚型，在 T 细胞受体刺激后，该亚型在外周 T 淋巴细胞中上调。这种激活诱导型同种型使用替代的第一个外显子 I.3 表达，它编码的蛋白质比普遍存在的 HIF-1 α 同种型长 24 个氨基酸。 HIF-1 α 的 mRNA 亚型 I.3 以组织特异性方式表达，在外周血白细胞和胸腺中表达最高。 (C) 2008 年美国组织相容性和免疫遗传学学会。由爱思唯尔公司出版。保留所有权利。
Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is critical not only in the regulation of oxygen homeostasis but also in the regulation of innate and adaptive immune systems. We previously reported that T-cell receptor-mediated activation of T cells in mice leads to the expression of an. alternative isoform of HIF-1 alpha that inhibits activated T cells in a delayed negative feed-back manner. In this paper, we describe a novel mRNA isoform of human HIF-1 alpha that is upregulated in peripheral T lymphocytes after T-cell receptor stimulation. This activation-inducible isoform is expressed using the alternative first exon I.3, and it encodes a protein that is 24 amino acids Longer than the ubiquitous HIF-1 alpha isoform. This mRNA isoform I.3 of HIF-1 alpha is expressed in a tissue-specific manner with the highest expression found in peripheral blood leukocytes and the thymus. (C) 2008 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.