Time-course imaging of rat embryos in utero with magnetic resonance microscopy
Time-course imaging of rat embryos in utero with magnetic resonance microscopy
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DOI：
10.1002/mrm.1910390424
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发表时间：
1998-04-01
影响因子：
3.3
通讯作者：
Johnson, GA
中科院分区：
文献类型：
--
作者：
Smith, BR;Shattuck, MD;Johnson, GA
磁共振（MR）显微镜用于无创地研究活大鼠胚胎在子宫内的发育情况。多年来，对发育中的哺乳动物胚胎进行连续观察的困难一直困扰着发育生物学家。大多数当前技术通过观察终点表型（在固定样本中）而不是研究活胚胎来分析正常和异常发育。 MR 显微镜经过改造，可以对每窝大鼠进行 3 次扫描（每隔 1 至 3 天），并生成活体发育胚胎的图像。结果表明，重复的麻醉和成像方案不会在随后交付和观察的幼鼠中产生明显的畸形。使用相位回卷器的三维投影编码在大约 30 分钟内产生了各向同性 (256(3)) 图像数据集，并且由于羊水中的稳态效应而产生了出色的组织对比度。
Magnetic resonance (MR) microscopy was used to noninvasively investigate the development of live rat embryos in utero. The difficulty in making sequential observations of a developing mammalian embryo has frustrated developmental biologists for many years. Most current technologies analyze normal and abnormal development by observing end point phenotypes (in fixed specimens) rather than investigating the live embryo. MR microscopy was adapted to allow rat litters to be scanned three times each (at 1- to 3-day intervals) and has produced images of live developing embryos. It was demonstrated that repeated anesthesia and imaging protocols produced no gross malformations in the rat pups that were subsequently delivered and observed. Three-dimensional projection encoding with phase rewinders produced isotropic (256(3)) image data sets in about 30 minutes with excellent tissue contrast arising from steady-state effects in the amniotic fluid.