Effect of Micropolyspora faeni cells and cell wall fractions on rabbit alveolar macrophages.
Effect of Micropolyspora faeni cells and cell wall fractions on rabbit alveolar macrophages.
复制标题
法氏微多孢菌细胞和细胞壁组分对兔肺泡巨噬细胞的影响。
DOI：
10.1128/iai.39.2.948-954.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
Snyder,IS
中科院分区：
文献类型：
--
作者：
Learn,DB;Snyder,IS
本文研究了肺泡巨噬细胞(AM)对费氏小多孢子菌细胞和细胞壁部分的反应性。培养的AM暴露于M.faeni及其CWF可使AM形成团块或聚集体，这些团块或聚集体仍然附着在培养皿表面。其他革兰氏阳性和革兰氏阴性细菌以及酵母菌、酵母多糖、乳胶微球和从单核细胞增生性李斯特菌分离的肽多聚糖不会引起这种反应。该反应与培养所用血清的种源和抗体含量无关。使用热灭活血清否定补体激活在AM聚集中的作用AM培养需要在接触细胞或CWF之前进行一段时间的培养，才能产生这种反应。这种反应具有时间和剂量依赖性。兔腹膜巨噬细胞也表现出聚集反应。用溶菌酶降解纯化的CWF，组分3，大大降低了聚集反应。用高碘酸盐、甲酰胺或三氯乙酸对组分3进行化学纯化也会降低这一活性。这些数据表明，引起这种反应的主要活性成分是肽聚糖，但与细胞壁相关的其他物质也可能是重要的。正常兔AM经Feni细胞和CWF处理后的培养液中有可溶性因子趋化作用，CWF也使正常兔AM化学发光。
The reactivity of alveolar macrophages (AM) to cells and cell wall fractions (CWF) of Micropolyspora faeni was investigated. Exposure of cultured AM to M. faeni and its CWF caused the AM to form clumps or aggregates which remained attached to the culture dish surface. Other gram-positive and gram-negative bacteria as well as yeast, zymosan, latex microspheres, and isolated peptidoglycan from Listeria monocytogenes did not cause this response. The response was independent of species source and antibody content of the serum used in culture. The use of heat-inactivated sera negated the role of complement activation in the aggregation of AM. AM cultures required a period of culture before exposure to cells or CWF for this response to occur. This response was both time and dose dependent. Rabbit peritoneal macrophages also exhibited the clumping response. Degradation of a purified CWF, fraction 3, with lysozyme greatly diminished the clumping response. Chemical purification of fraction 3 with periodate, formamide, or trichloracetic acid also decreased this activity. These data suggest that the major active component causing this response is peptidoglycan but that other materials associated with the cell wall may also be important. A soluble-factor chemotactic for normal rabbit AM was found in the culture fluid of AM exposed to fraction 3. M. faeni cells and CWF also caused normal rabbit AM to chemiluminesce.