Cell death and cell-cycle arrest induced by incorporation of [3H]thymidine into human haemopoietic cell lines.
Cell death and cell-cycle arrest induced by incorporation of [3H]thymidine into human haemopoietic cell lines.
复制标题
[3H]胸苷掺入人造血细胞系诱导细胞死亡和细胞周期停滞。
DOI：
10.1080/095530000138637
复制
发表时间：
2000
影响因子：
2.6
通讯作者：
H. Teraoka
中科院分区：
文献类型：
--
作者：
M. Yanokura;K. Takase;K. Yamamoto;H. Teraoka
目的
PURPOSE
To investigate the influence of [methyl-3H]thymidine ([3H]Tdr) incorporated into human haemopoietic cell lines.
MATERIALS AND METHODS
HL-60, Molt-4, Jurkat, Raji and SKW6-CL4 cells were incubated in the presence of [3H]Tdr. Cell proliferation, cell viability, DNA fragmentation and expression of caspase-3 and Bcl-2 families were examined. The cell-cycle of HL-60 was analysed using flow cytometry.
RESULTS
In HL-60, Molt-4 and Jurkat, cell death was accompanied by DNA nucleosomal fragmentation and activation of caspase-3. In Raji and SKW6-CL4, it was accompanied by neither. Protein levels of Bcl-2 and Bad in HL-60 and Molt-4 did not significantly change, and that of Bax was decreased after a 3-day incubation. HL-60 incubated in the presence of 74 or 185 kBq/ml [3H]Tdr arrested at G2/M phase, and then underwent apoptosis. In 7.4 kBq/ml, the cell-cycle progressed after the delay in S-phase.
CONCLUSIONS
Two different modes of cell death were observed when [3H]Tdr was incorporated into the human haemopoietic cell lines. Incorporation into HL-60 cells resulted in delay of S-phase progression, arrest at G2/M and apoptosis.