Orally administered OVA/CpG-ODN induces specific mucosal and systemic immune response in young and aged mice
Orally administered OVA/CpG-ODN induces specific mucosal and systemic immune response in young and aged mice
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DOI：
10.1189/jlb.0604330
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发表时间：
2005-06-01
影响因子：
5.5
通讯作者：
Pistoresi-Palencia, MC
中科院分区：
文献类型：
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作者：
Alignani, D;Maletto, B;Pistoresi-Palencia, MC
我们先前已经证明，皮下注射卵白蛋白(OVA)和合成的含有免疫刺激CpG基序的寡核苷酸(CpG-ODN)作为佐剂，可以刺激老龄小鼠的细胞和体液免疫，并促进T辅助细胞I型分化。本研究评价了CpG-ODN在幼龄(3月龄)和老年(18月龄)BALB/c小鼠口服免疫后诱导OVA特异性免疫应答的能力。口服OVA/CpG-ODN免疫在两组AGE中均可诱导相似的OVA特异性T细胞增殖反应(在粘膜和全身组织中)、血浆中的免疫球蛋白G(Ig G)和肠道冲洗液中的IgA。在老年小鼠中观察到的OVA特异性体液免疫反应与在年轻小鼠中观察到的相似，在最后一次口服免疫后第7天达到高峰，并在最后一次口服免疫后40天以上出现。两组小鼠的培养上清液中释放的细胞因子模式相似，在没有白细胞介素5反应的情况下具有特异性的干扰素-γ分泌。这些结果表明，口服OVA/CpG-ODN可诱导老年小鼠抗OVA的年轻、特异的免疫应答，提示CpG-ODN可作为衰老过程中的一种粘膜佐剂。
We have previously demonstrated that subcutaneously administered ovalbumin (OVA) plus synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs (CpG-ODN) as adjuvant stimulate cellular and humoral immunity and promote T helper cell type I differentiation in aged mice. The present study assessed the ability of CpG-ODN to induce an OVA-specific immune respouse after oral immunization in young (3-month-old) and aged (18-month-old) BALB/c mice. Oral OVA/CpG-ODN immunization induces a similar OVA-specific T cell-proliferative response (in mucosal and systemic tissues), immunoglobulin G (IgG) in plasma, and IgA in intestinal washes in both groups of ages. The OVA-specific humoral immune response observed in aged mice was similar to the one observed in young mice, peaking at day 7 after the last oral immunization and was present over 40 days after the last oral immunization. The pattern of cytokines released in culture supernatants in both groups of mice was similar, with specific interferon-gamma secretion in the absence of interleukin-5 responses. These results provide evidence that orally administered OVA/CpG-ODN induces a young-like, specific, immune response against OVA in aged mice, showing that CpG-ODN might be used as a mucosal adjuvant during aging.