In vitro alternatives to whole animal testing. Comparative cytotoxicity studies of divalent metals in established cell lines derived from tropical and temperate water fish species in a neutral red assay
In vitro alternatives to whole animal testing. Comparative cytotoxicity studies of divalent metals in established cell lines derived from tropical and temperate water fish species in a neutral red assay
复制标题
DOI：
10.1016/0141-1136(95)00058-5
复制
发表时间：
1996-06
影响因子：
3.3
通讯作者：
S. Magwood;S. George
中科院分区：
文献类型：
--
作者：
S. Magwood;S. George
用简单的活性染料（中性红）摄取法研究了7种二价金属阳离子对大菱鲆（TF）和蓝鳃鱼（BF-2）细胞系的急性毒性。该方法在每周的重复实验之间具有极高的可重复性，并且在不同的实验室之间具有可重复性。虽然金属的相对毒性存在一些物种依赖差异，但毒性与金属离子的化学柔软度（Σp）以及体内和体外毒性之间存在良好的相关性。这些研究表明，在96孔微孔板上进行的这种简单且相对便宜的技术可能对评价相关化合物基团之间的结构-毒性关系非常有用。
A simple vital dye (neutral red) uptake method for investigation of the acute toxicity of seven divalent metal cations to cell lines derived from the turbot (TF) and bluegill (BF-2) was evaluated. The method was extremely reproducible between replicate experiments on a week to week basis as well as being reproducible between different laboratories. Whilst there were some species-dependent differences in the relative toxicities of the metals there were good correlations between toxicity and the chemical softness (Σp) of the metal ions and also between in vivo and in vitro toxicities. These studies demonstrate that this simple and relatively inexpensive technique carried out in 96-well microplates may be extremely useful for evaluation of structure-toxicity relationships between related groups of compounds.