microRNAs control of in vivo toxicity from graphene oxide in Caenorhabditis elegans.
microRNAs control of in vivo toxicity from graphene oxide in Caenorhabditis elegans.
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DOI：
10.1016/j.nano.2014.04.005
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Qiuli Wu;Yunli Zhao;Gui Zhao;Dayong Wang
中科院分区：
文献类型：
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作者：
Qiuli Wu;Yunli Zhao;Gui Zhao;Dayong Wang
氧化葡萄糖烯(GO)毒性的分子基础仍很不清楚。在这里，我们使用秀丽线虫来研究microRNAs(MiRNAs)对GO毒性的控制。借助于固体测序，我们在GO暴露的线虫中鉴定了23个上调和8个下调的miRNAs。基因本体论和KEGG途径数据库分析表明，这些已鉴定的miRNAs可能参与了许多生物学过程的调控，其中一些提示了GO可能的新功能。已鉴定的miRNAs在调节GO对寿命的毒性方面的功能已在现有的miRNAs突变体中得到证实。此外，我们提供了证据，提出了GO可能通过影响受miRNAs控制的胰岛素/IGF信号、TOR信号和生殖系信号通路的功能而减少寿命的假说。我们的结果将有助于理解GO毒性的分子基础，并找到有效的表面修饰以减少GO毒性的线索。在本研究中，通过microRNA分析，研究了氧化石墨烯在秀丽线虫模型中的毒性。作者报道了GO影响多条重要通路，并提出GO可能通过影响胰岛素/IGF信号通路、TOR信号通路和生殖系信号通路的功能而减少寿命的假说。
The molecular basis forin vivographene oxide (GO) toxicity is still largely unclear. We here used Caenorhabditis elegans to investigate the microRNAs (miRNAs) control of GO toxicity. With the aid of SOLiD sequencing, we identified 23 up-regulated and 8 down-regulated miRNAs in GO-exposed nematodes. Gene ontology and KEGG pathway database analysis implied that these identified miRNAs might be involved in control of many biological processes, and some of them suggest the possible new functions of GO. Functions of the identified miRNAs in regulating the GO toxicity on lifespan were confirmed in the available miRNAs mutants. Moreover, we provide the evidence to raise a hypothesis that GO may reduce lifespan through influencing the functions of insulin/IGF signaling, TOR signaling, and germline signaling pathways controlled by miRNAs. Our results will be helpful for understanding the molecular basis for GO toxicity, and finding clues for useful surface modifications to reduce GO toxicity.From the Clinical EditorIn this study, toxicity of graphene oxide is studied in a Caenorhabditis elegans model via microRNA analysis. The authors report that multiple important pathways are influenced by GO and raise a hypothesis that GO may reduce lifespan through influencing the functions of insulin/IGF signaling, TOR signaling, and germline signaling pathways.