Quantitative Proteomics Analysis of Chondrogenic Differentiation of C3H10T1/2 Mesenchymal Stem Cells by iTRAQ Labeling Coupled with On-line Two-dimensional LC/MS/MS
Quantitative Proteomics Analysis of Chondrogenic Differentiation of C3H10T1/2 Mesenchymal Stem Cells by iTRAQ Labeling Coupled with On-line Two-dimensional LC/MS/MS
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通过 iTRAQ 标记结合在线二维 LC/MS/MS 对 C3H10T1/2 间充质干细胞的软骨分化进行定量蛋白质组学分析
DOI：
10.1074/mcp.m900243-mcp200
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发表时间：
2010-03-01
影响因子：
7
通讯作者：
Wu, Wei
中科院分区：
文献类型：
--
作者：
Ji, Yu-hua;Ji, Ju-ling;Wu, Wei
多能间充质干细胞（MSCs）的成软骨潜能使其成为软骨缺损细胞治疗的一个有希望的来源；然而，软骨形成的细胞内分子机制以及间充质干细胞的自我更新在很大程度上仍然未知。为了更深入地了解其潜在的分子机制，我们应用等压标签相对绝对定量（iTRAQ）标记结合在线二维LC/MS/MS技术鉴定体外软骨形成模型中差异表达的蛋白质：小鼠胚胎间充质细胞系C3H10T1/2细胞在微量培养和100 ng/ml骨形态发生蛋白2处理6天后诱导成软骨分化。共鉴定出1756个蛋白，平均错误发现率为0.45
The chondrogenic potential of multipotent mesenchymal stem cells (MSCs) makes them a promising source for cell-based therapy of cartilage defects; however, the exact intracellular molecular mechanisms of chondrogenesis as well as self-renewal of MSCs remain largely unknown. To gain more insight into the underlying molecular mechanisms, we applied isobaric tag for relative and absolute quantitation (iTRAQ) labeling coupled with on-line two-dimensional LC/MS/MS technology to identify proteins differentially expressed in an in vitro model for chondrogenesis: chondrogenic differentiation of C3H10T1/2 cells, a murine embryonic mesenchymal cell line, was induced by micromass culture and 100 ng/ml bone morphogenetic protein 2 treatment for 6 days. A total of 1756 proteins were identified with an average false discovery rate