TEMPERATURE AND SALT DEPENDENCE OF THE GEL MIGRATION ANOMALY OF CURVED DNA FRAGMENTS
TEMPERATURE AND SALT DEPENDENCE OF THE GEL MIGRATION ANOMALY OF CURVED DNA FRAGMENTS
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DOI：
10.1093/nar/15.1.247
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发表时间：
1987-01-12
影响因子：
14.9
通讯作者：
DIEKMANN, S
中科院分区：
文献类型：
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作者：
DIEKMANN, S
已经克隆了一系列不同序列的寡核苷酸来研究DNA弯曲。在10%聚丙烯酰胺凝胶中分析含有这些寡核苷酸的不同重复数的几个DNA片段。一个强大的凝胶迁移异常被发现为dA4序列，一个非常小的，但可以清楚地检测到的异常观察到dA3（都在重复长度为10个碱基对）。这些DNA片段的凝胶迁移异常的温度和盐（NaCl，MgCl 2）的依赖性进行了测量。虽然观察到类似的行为的所有序列的添加NaCl，温度和MgCl2依赖性的异常随寡核苷酸序列。这些数据被解释在由温度和MgCl2浓度的变化，影响弯曲的DNA片段的平面性引起的局部DNA结构的变化。
A series of oligonucleotides of different sequences have been cloned to study DNA curvature. Several DNA fragments containing these oligonucleotides in various numbers of repeats were analyzed in 10% polyacrylamide gels. A strong gel migration anomaly was found for dA4sequences; a comparably very small but clearly detectable anomaly was observed for dA3(both in a repeat length of 10 base-pairs). The temperature and salt (NaCl, MgCl2) dependence of the gel migration anomaly of these DNA fragments was measured. While a similar behaviour of all sequences is observed for the addition of NaCl, the temperature and MgCl2dependence of the anomaly varies with the oligonucleotide sequence. These data are interpreted in terms of local DNA structure changes induced by changes in the temperature and the MgCl2concentration which affect the planarity of the curved DNA fragments.