Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species
Transcriptomic profiling revealed key signaling pathways for cold tolerance and acclimation of two carp species
复制标题
转录组分析揭示了两种鲤鱼耐寒和驯化的关键信号通路
DOI：
10.1186/s12864-020-06946-8
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发表时间：
2020-08-05
期刊：
影响因子：
4.4
通讯作者：
Cui, Zongbin
中科院分区：
文献类型：
--
作者：
Ge, Guodong;Long, Yong;Cui, Zongbin
背景鲤鱼科（Cyprinidae）的近缘物种已经进化出在冷胁迫下独特的生存能力，但是产生抗寒性的分子机制仍然很大程度上未知。在这项研究中，我们比较了转录组谱的两种鲤鱼，以确定关键因素和途径的耐寒性和acquisition. ResultsResultsSongpu镜鲤和无倒刺鲤的幼虫，在18 ℃预处理24小时，显着提高其存活率在致死冷温度8 ℃或10 ℃，表明这两个鲤鱼物种具有冷驯化能力。但松浦镜鲤的耐寒能力和适应能力强于无须鲤。通过RNA-seq技术比较了松浦镜鲤和无须鲤仔鱼在28 ℃和18 ℃低温驯化过程中的转录组学特征。通过生物信息学分析和文氏图分析，筛选出与两种鲤鱼冷驯化差异密切相关的差异表达基因。GO富集分析表明，松浦镜鲤在冷驯化过程中上调最多的生物学过程是参与形态建成、次生醇代谢和药物转运的细胞组分组装。相反，宏观自噬，细胞内蛋白质转运，有机氮化合物催化剂的正调控是最下调的生物学过程中的冷驯化？KEGG富集分析表明，FoxO相关信号通路中的因子是造成两种鲤鱼耐寒性和适应性差异的主要原因，用抑制剂或激活剂改变FoxO相关信号通路中关键蛋白的磷酸化水平，可显著降低松浦镜鲤的耐寒性和适应性。这些数据提供了关键线索的解剖的分子机制的耐冷性和驯化在carps.ConclusionsThese研究结果表明，两种鲤鱼的幼虫具有不同的耐冷能力，可以建立在轻度低温冷驯化。多种生物学过程和FoxO相关信号通路与两种鲤鱼耐寒性和适应性差异的形成密切相关。
BackgroundClosely related species of the carp family (Cyprinidae) have evolved distinctive abilities to survive under cold stress, but molecular mechanisms underlying the generation of cold resistance remain largely unknown. In this study, we compared transcriptomic profiles of two carp species to identify key factors and pathways for cold tolerance and acclimation.ResultsLarvae of Songpu mirror carp and Barbless carp that were pretreated at 18 degrees C for 24h significantly improved their survival rates under lethal cold temperature at 8 degrees C or 10 degrees C, indicating that two carp species possess the ability of cold acclimation. However, Songpu mirror carp exhibited stronger abilities of cold tolerance and acclimation than Barbless carp. Transcriptomic profiles of Songpu mirror carp and Barbless carp larvae at 28 degrees C and 18 degrees C were compared during cold acclimation through RNA-seq. Differentially expressed genes that are closely associated with the differences in cold acclimation between two carp species were identified through bioinformatics and Venn's diagram analysis. GO enrichment analysis of these genes indicated that cellular component assembly involved in morphogenesis, secondary alcohol metabolism and drug transport were the most up-regulated biological processes during cold acclimation of Songpu mirror carp. Conversely, positive regulation of macroautophagy, intracellular protein transport, and organonitrogen compound catabolism were the most down-regulated biological processes during cold acclimation of Barbless carp. KEGG enrichment analysis revealed that factors in the FoxO-related signaling pathways are mainly responsible for the development of differences in cold tolerance and acclimation between two carp species since altering the phosphorylation of key proteins in the FoxO-related signaling pathways with inhibitors or an activator significantly decreased the cold tolerance and acclimation of Songpu mirror carp. These data provided key clues for dissection of molecular mechanisms underlying the development of cold tolerance and acclimation in carps.ConclusionsThese findings indicate that larvae of two carp species possess different abilities of cold tolerance and can build cold acclimation under mild low temperature. Multiple biological processes and FoxO-related signaling pathways are closely associated with the development of differences in cold tolerance and acclimation between two carp species.