PEP-19 immunoreactivity in the cochlear nucleus and superior olive of the cat.
PEP-19 immunoreactivity in the cochlear nucleus and superior olive of the cat.
复制标题
猫的耳蜗核和上橄榄核中的 PEP-19 免疫反应性。
DOI：
10.1016/s0306-4522(97)00407-7
复制
发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Spirou,GA
中科院分区：
文献类型：
--
作者：
Berrebi,AS;Spirou,GA
我们应用抗血清PEP-19，一个假定的钙结合多肽，猫的听觉脑干，以确定这种抗血清是否会选择性地显示耳蜗核神经元和他们的预测。我们报告说，整个人口的腹侧耳蜗核丛状和多极细胞，但不是章鱼细胞，表达这种肽在他们的胞体和树突。假定位于背侧的球形丛状细胞的轴突和位于腹侧的梯形体中的较厚的球形丛状细胞纤维被免疫染色，假定代表多极细胞轴突的细纤维也被免疫染色。大的内侧核的斜方体的肾盏末梢是密集的免疫反应，是较小的点状轮廓，概述免疫阴性神经元的配置文件在内侧和外侧上级橄榄。免疫标记的这些特征表明PEP-19在所有神经元隔室中表达。在整个上级橄榄复合体中，免疫标记的神经元相对较少，其中绝大多数位于橄榄周核中。有更多的免疫染色的神经元在外侧比内侧橄榄周细胞群，但他们的组合数量是相形见绌的免疫标记的细胞在腹侧耳蜗核的数量。PEP-19免疫反应阳性纤维和斑点的分布很好地定义了主核和一些橄榄周细胞群的边界。由于腹侧耳蜗核丛状细胞包括主要的输入到上级橄榄的主要核，和整个丛状细胞群免疫标记的PEP-19抗血清，其输入的数量和分布可以量化。在这项研究中，我们报告的免疫反应点并列的细胞体和近端树突的神经元在内侧上级橄榄出现的密度为20/100 μ m2。此外，我们表明，通过包埋前免疫电子显微镜，PEP-19-免疫反应性点状配置文件中观察到的内侧上级橄榄光镜表示突触前终末扣，包含圆形突触囊泡和形成不对称的突触连接，功能传统上与兴奋性突触。因此，这种抗血清代表了一个有用的工具，用于调查腹侧耳蜗核纤维和突触末梢内的目标核在上级橄榄的分布。
We applied antiserum to PEP-19, a presumptive calcium-binding polypeptide, to the auditory brainstem of cats to determine whether this antiserum would selectively reveal cochlear nucleus neurons and their projections. We report that the entire populations of ventral cochlear nucleus bushy and multipolar cells, but not octopus cells, express this peptide in their somata and dendrites. Presumed axons of spherical bushy cells located dorsally and thicker globular bushy cell fibers located ventrally in the trapezoid body are immunostained, as are thin fibers presumed to represent the axons of multipolar cells. Large calyceal endings in the medial nucleus of the trapezoid body are densely immunoreactive as are smaller punctate profiles that outline immunonegative neuronal profiles in the medial and lateral superior olives. These features of immunolabeling indicate that PEP-19 is expressed in all neuronal compartments. Within the entire superior olivary complex, relatively few neurons are immunolabeled, and the vast majority of these are found in the periolivary nuclei. There are many more immunostained neurons in lateral than in medial periolivary cell groups, but their combined numbers are dwarfed by the numbers of immunolabeled cells in the ventral cochlear nucleus. The borders of the principal nuclei and some of the periolivary cell groups are well defined by the distribution of PEP-19-immunoreactive fibers and puncta. Since ventral cochlear nucleus bushy cells comprise the predominant input to principal nuclei of the superior olive, and the entire bushy cell population is immunolabeled by PEP-19 antiserum, the numbers and distribution of their inputs can be quantified. In this study we report that immunoreactive puncta apposed to the cell bodies and proximal dendrites of neurons in the medial superior olive occur at a density of 20/100μm2. Moreover, we demonstrate by pre-embedding immunoelectron microscopy that the PEP-19-immunoreactive punctate profiles observed in the medial superior olive by light microscopy represent presynaptic terminal boutons that contain round synaptic vesicles and form asymmetric synaptic junctions, features traditionally associated with excitatory synapses. Thus, this antiserum represents a useful tool for investigating the distribution of ventral cochlear nucleus fibers and synaptic terminals within their target nuclei in the superior olive.