A CD14-independent LPS receptor cluster
A CD14-independent LPS receptor cluster
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DOI：
10.1038/86342
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发表时间：
2001-04-01
影响因子：
30.5
通讯作者：
Dedrick, RL
中科院分区：
文献类型：
--
作者：
Triantafilou, K;Triantafilou, M;Dedrick, RL
细菌脂多糖（LPS）是革兰氏阴性细菌外壁的主要结构成分，是炎症反应的有效引发剂，也是细菌感染的指标。尽管 CD14 已被确定为主要的 LPS 受体，但越来越多的证据表明可能存在其他功能性受体。在本研究中，利用亲和色谱、肽质量指纹图谱和荧光共振能量转移，我们鉴定了四种新蛋白，它们在LPS连接后形成激活簇并参与LPS信号转导。在这里，我们提供的证据表明热休克蛋白 70 和 90、趋化因子受体 4 和生长分化因子 5 是细菌脂多糖激活的主要介质。
Bacterial lipopolysaccharide (LPS), the major structural component of the outer wall of Gram-negative bacteria, is a potent initiator of an inflammatory response and serves as an indicator of bacterial infection. Although CD14 has been identified as the main LPS receptor, accumulating evidence has suggested the possible existence of other functional receptor(s). In this study, using affinity chromatography, peptide mass fingerprinting and fluorescence resonance energy transfer, we have identified four new proteins that form an activation cluster after LPS ligation and are involved in LPS signal transduction. Here we present evidence that implicates heat shock proteins 70 and 90, chemokine receptor 4 and growth differentiation factor 5 as the main mediators of activation by bacterial lipopolysaccharide.