Contribution of Energy Restriction and Macronutrient Composition to Changes in Adipose Tissue Gene Expression during Dietary Weight-Loss Programs in Obese Women
Contribution of Energy Restriction and Macronutrient Composition to Changes in Adipose Tissue Gene Expression during Dietary Weight-Loss Programs in Obese Women
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DOI：
10.1210/jc.2008-0814
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发表时间：
2008-11-01
影响因子：
5.8
通讯作者：
Langin, Dominique
中科院分区：
文献类型：
--
作者：
Capel, Frederic;Viguerie, Nathalie;Langin, Dominique
背景：低能量饮食被用于减少肥胖受试者的体脂量和代谢危险因素。脂肪组织中的分子变化与体重减轻有关，特别是与饮食组成有关，目前还知之甚少。目的：我们根据能量缺乏和饮食中的脂肪和碳水化合物含量，研究了人类肥胖妇女脂肪组织基因的表达。在8个欧洲临床中心招募的肥胖受试者进行了为期10周的低脂（高碳水化合物）或中等脂肪受试者：从648名受试者中选择两组，每组47名妇女，每个饮食臂中有47名妇女，这些受试者的人体测量和生物学参数相匹配。我们使用候选基因方法测量了一组脂肪组织基因表达的变化。另一组使用DNA微阵列调查了24，469份转录本。使用专用统计方法分析结果。在另一组受试者中证实了饮食敏感性调节。结果：两种饮食引起了相似的体重减轻，并且除了血脂谱成分之外，大多数生物学变量的变化也相似。一千个基因，被能量限制。我们验证了五个基因（FABP 4，NR 3C 1，SIRT 3，FNTA和GABARAPL 2）的脂肪与碳水化合物的比例与表达增加的影响在moderate-fat diet.Conclusions：能量限制有更明显的影响，在人类脂肪组织基因表达的变化比宏量营养素组成。大量营养素敏感的调节基因的一个子集可能会影响脂肪组织的功能和代谢反应。（临床内分泌代谢杂志93：4315-4322，2008）
Context: Hypoenergetic diets are used to reduce body fat mass and metabolic risk factors in obese subjects. The molecular changes in adipose tissue associated with weight loss and specifically related to the dietary composition are poorly understood.Objective: We investigated adipose tissue gene expression from human obese women according to energy deficit and the fat and carbohydrate content of the diet.Design and Setting: Obese subjects recruited among eight European clinical centers were followed up 10 wk of either a low-fat (high carbohydrate) or a moderate-fat (low carbohydrate) hypoenergetic diet.Subjects: Two sets of 47 women in each dietary arm were selected among 648 subjects matched for anthropometric and biological parameters.Main Outcome Measure: We measured adipose tissue gene expression changes in one set using a candidate gene approach. The other set was used to survey 24,469 transcripts using DNA microarrays. Results were analyzed using dedicated statistical methods. Diet-sensitive regulations were confirmed on the other set of subjects.Results: The two diets induced similar weight loss and similar changes for most of the biological variables except for components of the blood lipid profile. One thousand genes were regulated by energy restriction. We validated an effect of the fat to carbohydrate ratio for five genes (FABP4, NR3C1, SIRT3, FNTA, and GABARAPL2) with increased expression during the moderate-fat diet.Conclusions: Energy restriction had a more pronounced impact on variations in human adipose tissue gene expression than macronutrient composition. The macronutrient-sensitive regulation of a subset of genes may influence adipose tissue function and metabolic response. (J Clin Endocrinol Metab 93: 4315-4322, 2008)