Determining Zygosity in the Vietnam Era Twin Registry: An Update
Determining Zygosity in the Vietnam Era Twin Registry: An Update
复制标题
DOI：
10.1375/twin.13.5.461
复制
发表时间：
2010-10-01
影响因子：
0.9
通讯作者：
Smith, Nicholas L.
中科院分区：
文献类型：
--
作者：
Forsberg, Christopher W.;Goldberg, Jack;Smith, Nicholas L.
我们的工作评估了越南时代双胞胎（VET）注册表中原始合子型分类的准确性，使用了一部分参与者的DNA标记的新信息。然后，我们构建了一个更新的接合性分类算法。VET登记册包括7，375名在越南时期服役的男性-男性双胞胎。在20世纪80年代中期，4,774对双胞胎完成了一份包含20个项目的合子型问卷。此外，还提供了包括血型在内的军事记录信息。在原始的接合性分类中使用了接合性问卷和血型中的项目。在1990 - 2009年间，从612对双胞胎中获得了DNA，并使用同卵双胞胎之间的一致性来分类合子。接下来，使用逻辑回归来构建预测的接合性概率，使用来自接合性问卷的项目与该子样本。根据新的卵性预测模型对所有双胞胎进行重新分类，并与原始的卵性分配进行比较。原始和新的预测的接合性概率高度相关（r = 0.962）和一致性的接合性分类同样高（Kappa = 0.936）。在最初的卵性分配错误主要是由于单卵双胞胎被误分类为双卵的基础上军事记录血型数据。删除军事记录血型数据显着提高了原始分类的准确性。基于接合性问卷的接合性分配对基于DNA的接合性具有高度预测性。从行政数据、军事记录或其他记录中扩充这种接合性分类，应谨慎行事。
Our work assessed the accuracy of the original zygosity classification in the Vietnam Era Twin (VET) Registry using new information from DNA markers on a subset of participants. We then constructed an updated zygosity classification algorithm. The VET Registry includes 7,375 male-male twin pairs who served in the military during the Vietnam era. During the mid-1980s 4,774 twin pairs completed a zygosity questionnaire of 20 items. Additionally, military record information, including blood group, was available. Items from the zygosity questionnaire and blood group were used in the original zygosity classification. Between 1990-2009 DNA was obtained from 612 twin pairs and concordance between co-twins was used to classify zygosity. Next logistic regression was used to construct predicted probabilities of zygosity using items from the zygosity questionnaire with this subsample. All twins were reclassified according to the new zygosity prediction model and compared with the original zygosity assignment. The original and new predicted probabilities of zygosity were highly correlated (r = 0.962) and concordance for the classification of zygosity was similarly high (kappa = 0.936). Errors in the original zygosity assignment were primarily due to monozygotic twins that were misclassified as dizygotic based on military record blood group data. Removing the military record blood group data markedly improved the accuracy of the original classification. Zygosity assignment based on a zygosity questionnaire was highly predictive of DNA-based zygosity. Augmentation of such a zygosity classification from administrative data, military records, or other records, should be done with caution.