Carbon nanotube-modified microelectrodes for simultaneous detection of dopamine and serotonin in vivo
Carbon nanotube-modified microelectrodes for simultaneous detection of dopamine and serotonin in vivo
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DOI：
10.1039/b705552h
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发表时间：
2007-01-01
期刊：
影响因子：
4.2
通讯作者：
Venton, B. Jill
中科院分区：
文献类型：
--
作者：
Swamy, B. E. Kumara;Venton, B. Jill
多巴胺和血清素是重要的神经递质，在大脑中相互作用。虽然多巴胺很容易用电化学传感器检测到，但血清素的检测更困难，因为氧化后形成的反应性物质可以吸附到电极上，降低灵敏度。电极的碳纳米管处理已经用于增加灵敏度、促进电子转移和减少结垢。大多数方法都集中在大电极的纳米管涂层和不利于体内测量的较慢的电化学技术上。在这项研究中，我们研究了单壁碳纳米管修饰的碳纤维微电极在体内多巴胺和5-羟色胺的共同检测。使用快速扫描循环伏安法，纳米管涂层后的神经递质的S/N比增加。纳米管的电催化效应在快速扫描速率下不明显，但在较慢的扫描速率下观察到更快的动力学。纳米管修饰的微电极显示出显着较少的污染后，暴露于血清素比裸电极。纳米管修饰的电极被用来监测刺激多巴胺和5-羟色胺的变化，同时在麻醉大鼠的纹状体后，给药的5-羟色胺合成前体。这些研究表明，纳米管涂层的微电极可以与快速扫描技术一起使用，并且由于其更高的灵敏度和抗污染性而有利于神经递质的体内测量。
Dopamine and serotonin are important neurotransmitters that interact in the brain. While dopamine is easily detected with electrochemical sensors, the detection of serotonin is more difficult because reactive species formed after oxidation can adsorb to the electrode, reducing sensitivity. Carbon nanotube treatments of electrodes have been used to increase the sensitivity, promote electron transfer, and reduce fouling. Most methods have focused on nanotube coatings of large electrodes and slower electrochemical techniques that are not conducive to measurements in vivo. In this study, we investigated carbon-fiber microelectrodes modified with single-walled carbon nanotubes for the co-detection of dopamine and serotonin in vivo. Using fast-scan cyclic voltammetry, S/N ratios for the neurotransmitters increased after nanotube coating. Electrocatalytic effects of nanotubes were not apparent at fast scan rates but faster kinetics were observed with slower scanning. Nanotube-modified microelectrodes showed significantly less fouling after exposure to serotonin than bare electrodes. The nanotube-modified electrodes were used to monitor stimulated dopamine and serotonin changes simultaneously in the striatum of anesthetized rat after administration of a serotonin synthetic precursor. These studies show that nanotube-coated microelectrodes can be used with fast scanning techniques and are advantageous for in vivo measurements of neurotransmitters because of their greater sensitivity and resistance to fouling.