beta-Catenin controls cell sorting at the notochord-somite boundary independently of cadherin-mediated adhesion.
beta-Catenin controls cell sorting at the notochord-somite boundary independently of cadherin-mediated adhesion.
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DOI：
10.1083/jcb.200503009
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发表时间：
2005-08-15
影响因子：
7.8
通讯作者：
Fagotto, Francois
中科院分区：
文献类型：
--
作者：
Reintsch, Wolfgang E;Habring-Mueller, Anette;Wang, Renee W;Schohl, Anne;Fagotto, Francois
在非洲爪蟾中，躯干中胚层到背侧脊索和侧体节的模式化依赖于Wnt-β-catenin信号传导的差异调节。为了研究这些组织的物理分离的细胞要求，我们操纵分散在树干中胚层内的单个细胞中的β-连环蛋白活性。我们发现高活性导致从脊索到体节的有效细胞分选，而降低活性导致相反方向的分选。对过度表达β-连环蛋白的单个细胞的分析显示，这些细胞不能与脊索细胞建立稳定的接触，但可以自由地穿过边界整合到体细胞组织中。干扰钙粘蛋白介导的粘附破坏组织结构，但它不影响分选和边界形成。基于这些结果，我们提出，边界本身是细胞自主的接触行为的变化，不依赖于绝对水平的粘附差异的结果。
In Xenopus laevis, patterning of the trunk mesoderm into the dorsal notochord and lateral somites depends on differential regulation of Wnt–β-catenin signaling. To study the cellular requirements for the physical separation of these tissues, we manipulated β-catenin activity in individual cells that were scattered within the trunk mesoderm. We found that high activity led to efficient cell sorting from the notochord to the somites, whereas reduced activity led to sorting in the opposite direction. Analysis of individual cells overexpressing β-catenin revealed that these cells were unable to establish stable contacts with notochord cells but could freely cross the boundary to integrate within the somitic tissue. Interference with cadherin-mediated adhesion disrupted tissue architecture, but it did not affect sorting and boundary formation. Based on these results, we propose that the boundary itself is the result of cell-autonomous changes in contact behavior that do not rely on differences in absolute levels of adhesion.